Nayak S S, Pattabiraman T N
Clin Chim Acta. 1981 Feb 5;109(3):267-74. doi: 10.1016/0009-8981(81)90312-0.
A new colorimetric method, based on the phenol sulphuric acid reaction of carbohydrates, is described for the determination of glycosylated hemoglobin. Hemolyzates were treated with 1 mol/l oxalic acid in 2 mol/l Hcl for 4 h at 100 degrees C, the protein was precipitated with trichloroacetic acid, and the free sugars and hydroxymethyl furfural in the protein free supernatant were treated with phenol and sulphuric acid to form the color. The new method is compared to the thiobarbituric acid method and the ion-exchange chromatographic method for the estimation of glycosylated hemoglobin in normals and diabetics. The increase in glycosylated hemoglobin in diabetic patients as estimated by the phenol-sulphuric acid method was more significant (P less than 0.001) than the increase observed by the thiobarbituric acid method (P less than 0.01). The correlation between the phenol-sulphuric acid method and the column method was better (r = 0.91) than the correlation between the thiobarbituric acid method and the column method (r = 0.84). No significant correlation between fasting and postprandial blood sugar level and glycosylated hemoglobin level as determined by the two colorimetric methods was observed in diabetic patients.
本文描述了一种基于碳水化合物的苯酚硫酸反应的新型比色法，用于测定糖化血红蛋白。将溶血产物在100℃下用2mol/L盐酸中的1mol/L草酸处理4小时，用三氯乙酸沉淀蛋白质，然后用苯酚和硫酸处理无蛋白上清液中的游离糖和羟甲基糠醛以形成颜色。将该新方法与硫代巴比妥酸法和离子交换色谱法进行比较，用于评估正常人和糖尿病患者的糖化血红蛋白。通过苯酚 - 硫酸法估计的糖尿病患者糖化血红蛋白的增加比硫代巴比妥酸法观察到的增加更显著（P <0.001）（P <0.01）。苯酚 - 硫酸法与柱法之间的相关性（r = 0.91）优于硫代巴比妥酸法与柱法之间的相关性（r = 0.84）。在糖尿病患者中，两种比色法测定的空腹和餐后血糖水平与糖化血红蛋白水平之间未观察到显著相关性。
