Commander Nicola J, Spencer Stephen A, Wren Brendan W, MacMillan Alastair P
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
Vaccine. 2007 Jan 2;25(1):43-54. doi: 10.1016/j.vaccine.2006.07.046. Epub 2006 Aug 4.
Five candidate genes from the Brucella melitensis 16M genome were selected. Eukaryotic expression plasmids encoding these antigens were constructed and expression was verified in vitro from transfected Cos7 cells. Each vaccine was assessed for protective efficacy in a BALB/c mouse brucellosis infection model. From these experiments two protective DNA vaccines were identified: p-omp25 and p-ialB. The Omp25 antigen (BMEI1249) has previously been studied in terms of Brucella virulence, serodiagnosis and as a protective antigen. However, this study represents the first report of a significant protective effect achieved against B. melitensis 16M challenge using the Omp25 antigen in a DNA vaccine approach. The other protective vaccine identified in this study was p-ialB. The ialB candidate (BMEI1584) was selected based upon its' putative function as an invasion protein which was assigned due to shared identity with the invasion protein B (ialB) of Bartonella bacilliformis. This candidate has not previously been investigated with regard to Brucella virulence or pathogenesis. This study is the first report to identify the Brucella invasion protein B (BMEI1584) as a novel protective antigen for brucellosis.
从羊种布鲁氏菌16M基因组中选择了5个候选基因。构建了编码这些抗原的真核表达质粒，并在转染的Cos7细胞中进行了体外表达验证。在BALB/c小鼠布鲁氏菌病感染模型中评估了每种疫苗的保护效力。通过这些实验鉴定出两种具有保护作用的DNA疫苗：p-omp25和p-ialB。Omp25抗原（BMEI1249）此前已在布鲁氏菌毒力、血清学诊断以及作为保护性抗原方面进行过研究。然而，本研究是首次报道使用Omp25抗原通过DNA疫苗方法对羊种布鲁氏菌16M攻击产生显著保护作用。本研究中鉴定出的另一种保护性疫苗是p-ialB。ialB候选基因（BMEI1584）是基于其作为侵袭蛋白的假定功能而选择的，该功能是由于与杆状巴尔通体的侵袭蛋白B（ialB）具有共同的同源性而赋予的。该候选基因此前尚未在布鲁氏菌毒力或发病机制方面进行过研究。本研究是首次报道将布鲁氏菌侵袭蛋白B（BMEI1584）鉴定为布鲁氏菌病的一种新型保护性抗原。
