Hérent Marie-France, De Bie Véronique, Tilquin Bernard
Unité d'Analyse Chimique et Physico-chimique des Médicaments, Université Catholique de Louvain, UCL 72.30, Avenue Emmanuel Mounier, 72, B-1200 Bruxelles, Belgium.
J Pharm Biomed Anal. 2007 Feb 19;43(3):886-92. doi: 10.1016/j.jpba.2006.09.005. Epub 2006 Oct 17.
The classical methods of chromatographic identification of compounds were based on calculation of retention indices by using different stationary phases. The aim of the work was to differentiate essential oils extracted from different plant species by identification of some of their major compounds. The method of identification was based on the calculation of new retention indices of essential oils compounds fractionated on a polar chromatographic column with temperature programming system. Similar chromatograms have been obtained on the same column for one plant family with two different temperature gradients allowing the rapid identification of essential oils of different species, sub-species or chemotypes of Citrus, Mentha and Thymus.
化合物色谱鉴定的经典方法是基于使用不同固定相计算保留指数。这项工作的目的是通过鉴定某些主要化合物来区分从不同植物物种中提取的香精油。鉴定方法基于在带有程序升温系统的极性色谱柱上对香精油化合物进行分馏后计算新的保留指数。对于同一植物科的两种不同温度梯度，在同一色谱柱上获得了相似的色谱图，从而能够快速鉴定柑橘属、薄荷属和百里香属不同物种、亚种或化学型的香精油。
