Zeng Ying-Xu, Zhao Chen-Xi, Liang Yi-Zeng, Yang Hong, Fang Hong-Zhuang, Yi Lun-Zhao, Zeng Zhong-Da
Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
Anal Chim Acta. 2007 Jul 9;595(1-2):328-39. doi: 10.1016/j.aca.2006.12.022. Epub 2006 Dec 19.
The volatile components between stems and roots and also among five Clematis species from China were studied and analyzed by gas chromatography-mass spectrometry (GC-MS) combined with alternative moving window factor analysis (AMWFA), a new chemometric resolution method. Identification of the compounds was also assisted by comparison of temperature-programmed retention indices (PTRIs) on HP-5MS with authentic samples included in our own laboratory database under construction. A total of 153 different compounds accounting for 86.6-96.5% were identified and significant qualitative and quantitative differences were observed among the samples. The major volatile components in different essential oils from Clematis species were n-hexadecanoic acid and (Z,Z)-9,12-octadecadienoic acid. Our work further demonstrated chemometric resolution techniques upon the two-dimensional data and PTRIs can provide a complementary and convenient method for fast and accurate analysis of complex essential oils.
采用气相色谱-质谱联用（GC-MS）结合一种新的化学计量学解析方法——交替移动窗口因子分析（AMWFA），对中国产5种铁线莲属植物茎与根之间以及不同种之间的挥发性成分进行了研究和分析。通过将HP-5MS上的程序升温保留指数（PTRIs）与我们正在构建的实验室数据库中的 authentic样品进行比较，也有助于化合物的鉴定。共鉴定出153种不同的化合物，占总量的86.6 - 96.5%，且样品之间存在显著的定性和定量差异。铁线莲属植物不同挥发油中的主要挥发性成分是正十六烷酸和（Z,Z）-9,12-十八碳二烯酸。我们的工作进一步证明，基于二维数据和PTRIs的化学计量学解析技术可以为快速、准确地分析复杂挥发油提供一种互补且便捷的方法。
