Lu J-C, Xu H-R, Chen F, Huang Y-F, Lu N-Q
Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, PR China.
Arch Androl. 2006 Nov-Dec;52(6):447-53. doi: 10.1080/01485010600822705.
We attempted to provide an instruction for the standardization of alpha-glucosidase level determination and quality controls within the same laboratory and among different laboratories. Each of 51 semen samples was divided into two aliquots, centrifuging at 1000 g for 10 min or 3000 g for 15 min. The alpha-glucosidase level and sperm concentration of each sample were assayed. The alpha-glucosidase level in seminal plasma obtained at 3000 g for 15 min centrifugation was significantly lower than that at 1000 g for 10 min (p = 0.001). An additional 6 samples of seminal plasma with or without phenylmethylsulfonyl fluoride (PMSF), obtained at 3000 g for 15 min centrifugation, were frozen for 20 days, and each of 6 samples was determined for their alpha-glucosidase levels after thawing every other day. There was no significant difference between alpha-glucosidase levels in seminal plasma regardless of the presence of PMSF. The alpha-glucosidase level increased with the length of abstinence period. In conclusion, centrifugal velocity and abstinence time should be standardized in the alpha-glucosidase determination. Frozen seminal plasma may serve as the sample for quality control among clinical laboratories.
我们试图为同一实验室内部以及不同实验室之间α-葡萄糖苷酶水平测定及质量控制的标准化提供指导。51份精液样本每份均分为两份，分别以1000 g离心10分钟或3000 g离心15分钟。对每份样本的α-葡萄糖苷酶水平和精子浓度进行测定。以3000 g离心15分钟所获精浆中的α-葡萄糖苷酶水平显著低于以1000 g离心10分钟所获精浆中的α-葡萄糖苷酶水平（p = 0.001）。另外6份以3000 g离心15分钟所获的添加或未添加苯甲基磺酰氟（PMSF）的精浆样本被冷冻20天，每隔一天解冻一份样本并测定其α-葡萄糖苷酶水平。无论是否存在PMSF，精浆中的α-葡萄糖苷酶水平均无显著差异。α-葡萄糖苷酶水平随禁欲期时长增加而升高。总之，在α-葡萄糖苷酶测定中应规范离心速度和禁欲时间。冷冻精浆可作为临床实验室间质量控制的样本。
