Breindl M, Holland J J
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2545-9. doi: 10.1073/pnas.72.7.2545.
The virion transcriptase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) of vesicular stomatitis virus was fully active when ribonucleoprotein cores from purified virions were added to cell-free protein synthesizing systems of eukaryotic origin. Synthesis of mRNA was linear for at least 3 hr and the newly synthesized viral mRNA was efficiently utilized for the synthesis of viral proteins N (nucleoprotein), NS, and M (matrix); small amounts of a putative G (glycoprotein protein precursor and several unidentified polypeptides were regularly synthesized. The ratio of the various newly synthesized viral proteins was identical after different periods of coupled mRNA and protein synthesis. Identical proteins were obtained when the cell-free protein synthesizing systems were programmed with purified VSV mRNA synthesized in vitro. No detectable L protein was synthesized, even though transcripts complementary to the complete viral genome were detectable in the mRNA preparation by hybridization.
当将来自纯化病毒粒子的核糖核蛋白核心添加到真核来源的无细胞蛋白质合成系统中时，水疱性口炎病毒的病毒粒子转录酶（核苷三磷酸：RNA核苷酸转移酶，EC 2.7.7.6）具有完全活性。mRNA的合成至少3小时呈线性，新合成的病毒mRNA被有效地用于合成病毒蛋白N（核蛋白）、NS和M（基质）；少量假定的G（糖蛋白前体）和几种未鉴定的多肽也经常被合成。在不同时期的mRNA和蛋白质偶联合成后，各种新合成的病毒蛋白的比例相同。当用体外合成的纯化VSV mRNA对无细胞蛋白质合成系统进行编程时，可获得相同的蛋白质。即使通过杂交在mRNA制剂中可检测到与完整病毒基因组互补的转录本，但未检测到L蛋白的合成。
