Neugebauer K M, Reichardt L F
Howard Hughes Medical Institute, San Francisco, California 94143-0724.
Nature. 1991 Mar 7;350(6313):68-71. doi: 10.1038/350068a0.
Integrins are a family of alpha beta heterodimeric receptors that mediate cell-cell and cell-substratum interactions. Integrin binding to extracellular ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. Mechanisms that regulate integrin function are, therefore, central to the participation of integrins in a diverse set of cellular events. Here we report the identification of TASC, a monoclonal antibody to a novel epitope on the integrin beta 1 subunit, which inhibits cell adhesion to vitronectin but promotes adhesion to laminin and collagen types I and IV. We show that developing retinal neurons that have lost responsiveness to laminin regain the ability to bind laminin in the presence of TASC. Thus, beta 1-class integrins are likely to occupy multiple affinity states that can be modulated at the cell surface.
整合素是一类αβ异二聚体受体家族，介导细胞间和细胞与基质的相互作用。整合素与细胞外配体的结合调节细胞黏附、形态、运动、细胞内信号传导和基因表达。因此，调节整合素功能的机制对于整合素参与多种细胞事件至关重要。在此，我们报告了TASC的鉴定，TASC是一种针对整合素β1亚基上一个新表位的单克隆抗体，它抑制细胞与玻连蛋白的黏附，但促进细胞与层粘连蛋白以及I型和IV型胶原蛋白的黏附。我们发现，对层粘连蛋白失去反应性的发育中的视网膜神经元在TASC存在的情况下恢复了结合层粘连蛋白的能力。因此，β1类整合素可能占据多种可在细胞表面调节的亲和状态。
