Lautz S, Kanbar T, Alber J, Lämmler C, Weiss R, Prenger-Berninghoff E, Zschöck M
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Str. 107, 35392 Giessen, Germany.
J Vet Med B Infect Dis Vet Public Health. 2006 Nov;53(9):434-8. doi: 10.1111/j.1439-0450.2006.00999.x.
Phenotypic properties and species-specific PCR tests based on the nuc gene of Staphylococcus intermedius and S. aureus, and a conserved region of 16S rDNA were used to identify 45 S. intermedius and four S. aureus isolated from samples of dogs during routine diagnostics. Four S. pseudintermedius strains used for control purposes reacted positively with the S. intermedius nuc PCR showing the close relationship between both species. Investigating the 45 S. intermedius and four S. pseudintermedius strains for the prevalence of the exfoliative toxin SIET encoding gene yielded the presence of the gene for 21 of the S. intermedius and two of the S. pseudintermedius strains. Partial sequencing of the toxin gene of a single S. intermedius strain and comparing this sequence with that obtained from GenBank revealed an almost complete identity. The presence of the exfoliative toxin gene could mainly be found among S. intermedius isolated from skin and wound infections and from otitis externa possibly indicating a role of this toxin for the clinical symptoms.
基于中间葡萄球菌和金黄色葡萄球菌的nuc基因以及16S rDNA保守区域的表型特性和种特异性PCR检测，用于在常规诊断过程中鉴定从犬类样本中分离出的45株中间葡萄球菌和4株金黄色葡萄球菌。用于对照目的的4株伪中间葡萄球菌菌株与中间葡萄球菌nuc PCR呈阳性反应，表明这两个物种之间关系密切。对45株中间葡萄球菌和4株伪中间葡萄球菌菌株进行剥脱毒素SIET编码基因流行率调查，结果显示21株中间葡萄球菌和2株伪中间葡萄球菌菌株存在该基因。对单个中间葡萄球菌菌株的毒素基因进行部分测序，并将该序列与从GenBank获得的序列进行比较，发现几乎完全相同。剥脱毒素基因主要存在于从皮肤和伤口感染以及外耳道炎分离出的中间葡萄球菌中，这可能表明该毒素在临床症状中起作用。
