Capsey L J, Williamson D H, Banks G R
Genetics Division, National Institute for Medical Research, Mill Hill, London, England.
Curr Genet. 1990 Dec;18(6):485-91. doi: 10.1007/BF00327018.
Electron microscopic analysis of thin sections of Saccharomyces cerevisiae NCYC74 has revealed the presence of many clumped cytoplasmic particles that morphologically resemble Ty element virus-like particles (VLPs). Accumulation of Ty VLPs has only previously been observed in S. cerevisiae strains that over-express a cloned Ty element. The particles in NCYC74 co-purify with Ty RNA, Ty-specific antigens and a reverse transcriptase activity. Furthermore, they appear to be recognized by antibodies to Ty VLPs during indirect immunofluorescence experiments. These observations provide compelling evidence that the cytoplasmic particle in NCYC74 are indeed Ty VLPs.
对酿酒酵母NCYC74薄切片的电子显微镜分析显示，存在许多聚集的细胞质颗粒，其形态类似于Ty元件病毒样颗粒（VLP）。此前仅在过表达克隆Ty元件的酿酒酵母菌株中观察到Ty VLP的积累。NCYC74中的颗粒与Ty RNA、Ty特异性抗原和逆转录酶活性共同纯化。此外，在间接免疫荧光实验中，它们似乎能被抗Ty VLP的抗体识别。这些观察结果提供了令人信服的证据，表明NCYC74中的细胞质颗粒确实是Ty VLP。
