Aves S J, Durkacz B W, Carr A, Nurse P
EMBO J. 1985 Feb;4(2):457-63. doi: 10.1002/j.1460-2075.1985.tb03651.x.
The cdc10 'start' gene from the fission yeast Schizosaccharomyces pombe has been cloned by rescue of mutant function. It is present as a single copy in the haploid genome. Hybridisation of the gene to Northern blots has identified a low abundance 2.7-kb polyadenylated RNA. Study of RNA extracted from cells both entering stationary phase and undergoing synchronous cell divisions suggests that commitment to the cell cycle is not controlled by regulation of cdc10 transcript level. DNA sequence analysis of the gene has identified an open reading frame capable of encoding a protein of mol. wt. 85 400. The putative cdc10 gene product shows no significant primary structure similarity with products of other fission and budding yeast cell cycle genes, or with other protein sequences in several databases.
通过突变功能拯救，已克隆出裂殖酵母粟酒裂殖酵母的cdc10“起始”基因。它在单倍体基因组中以单拷贝形式存在。该基因与Northern印迹杂交鉴定出一种低丰度的2.7 kb多聚腺苷酸化RNA。对进入稳定期和进行同步细胞分裂的细胞中提取的RNA的研究表明，细胞周期的进入不受cdc10转录水平调控的控制。该基因的DNA序列分析确定了一个能够编码分子量为85400的蛋白质的开放阅读框。推测的cdc10基因产物与其他裂殖和芽殖酵母细胞周期基因的产物，或几个数据库中的其他蛋白质序列没有明显的一级结构相似性。
