Wahab Z A, Wright G L
Int J Cancer. 1985 Dec 15;36(6):677-83. doi: 10.1002/ijc.2910360610.
The reactivity of monoclonal antibody (MAb) anti-leu 7 (HNK-1) on formalin-fixed sections of prostate tissues was determined by an immunoperoxidase assay. Anti-Leu 7 was found to react with both primary and metastatic tissues from 6/6 normal prostate, 15/15 benign prostatic hyperplasia, and 37/39 prostate carcinoma samples. Anti-Leu-7 reactivity was localized in the cytoplasm of the supranuclear region of prostatic epithelial cells and the fibromuscular stroma did not stain. Myelinated nerve fibers in the stroma were stained with anti-Leu 7 and this served as an internal control. Anti-Leu 7 did not bind to prostatic acid phosphatase (PAP) or prostate-specific antigen (PSA) in direct or competitive binding radioimmunoassays. Anti-Leu 7 was more effective (5/5) in the identification of metastatic tumors of prostate origin than an MAb to PSA (2/5). The differential pattern of reactivity of anti-Leu 7 compared to anti-PSA on serial sections of prostate tissues suggests that Leu 7 may be a useful diagnostic and prognostic marker of prostate cancer.
通过免疫过氧化物酶测定法，确定了单克隆抗体（MAb）抗-Leu 7（HNK-1）在前列腺组织福尔马林固定切片上的反应性。发现抗-Leu 7与6/6例正常前列腺、15/15例良性前列腺增生和37/39例前列腺癌样本的原发组织和转移组织均发生反应。抗-Leu-7反应性定位于前列腺上皮细胞核上区域的细胞质中，纤维肌性基质不着色。基质中的有髓神经纤维用抗-Leu 7染色，这作为内部对照。在直接或竞争性结合放射免疫测定中，抗-Leu 7不与前列腺酸性磷酸酶（PAP）或前列腺特异性抗原（PSA）结合。在识别前列腺来源的转移瘤方面，抗-Leu 7（5/5）比抗-PSA单克隆抗体（2/5）更有效。与抗-PSA相比，抗-Leu 7在前列腺组织连续切片上的不同反应模式表明，Leu 7可能是前列腺癌有用的诊断和预后标志物。
