Brown J L, Brunjes P C
Department of Psychology, University of Virginia, Charlottesville 22903.
J Comp Neurol. 1990 Nov 1;301(1):15-22. doi: 10.1002/cne.903010103.
The development of a second order structure in the olfactory pathway, the anterior olfactory nucleus, was examined in both normal rat pups and in subjects which underwent unilateral naris closure on postnatal day 1 (P1). Naris occlusion in neonatal rats produces a constellation of changes within the first relay in the pathway, the olfactory bulb, including a 25% reduction in total volume. Such large changes suggest that higher order structures might also be affected. Anterior olfactory nucleus development was quantified in several ways. Laminar volumes were computed by using serial section planimetry. In control animals differential development was observed, with regions extending most rostrally (e.g., pars externa and pars lateralis) exhibiting the least growth. The anterior olfactory nucleus on the "deprived" side of subjects with a single naris occluded was identical in size to that observed in controls, development within the pars lateralis was examined in control animals at P10, P20, P30, and adults. Developmental increases in numbers of both branches per cell and spines were noted, but mean branch length remained relatively constant. Finally, the effects of naris occlusion on histological patterns of succinate dehydrogenase (SDH) staining and 2-deoxyglucose uptake within pars lateralis were examined at P20 to test for more subtle effects of naris occlusion. SDH staining was quite similar in deprived and control rats at P20. However, 3H-2-DG uptake was decreased in rostral areas of the anterior olfactory nucleus ipsilateral to the deprived olfactory bulb, suggesting that naris closure does affect the structure.
在正常幼鼠和出生后第1天（P1）接受单侧鼻孔封闭的实验对象中，研究了嗅觉通路中二级结构——前嗅核的发育情况。新生大鼠的鼻孔闭塞会在嗅觉通路的第一级中继站——嗅球内引发一系列变化，包括总体积减少25%。如此大的变化表明更高阶的结构可能也会受到影响。前嗅核的发育通过多种方式进行量化。通过连续切片平面测量法计算层体积。在对照动物中观察到了差异发育，最靠前延伸的区域（如外侧部和外侧 pars）生长最少。单侧鼻孔闭塞的实验对象“受剥夺”一侧的前嗅核大小与对照中观察到的相同，在P10、P20、P30和成年期对对照动物的外侧部发育进行了研究。注意到每个细胞的分支数和棘突数量在发育过程中增加，但平均分支长度保持相对恒定。最后，在P20时检查了鼻孔闭塞对外侧部琥珀酸脱氢酶（SDH）染色组织学模式和2-脱氧葡萄糖摄取的影响，以测试鼻孔闭塞的更细微影响。P20时，受剥夺大鼠和对照大鼠的SDH染色非常相似。然而，与受剥夺嗅球同侧的前嗅核 Rostral 区域的3H-2-DG摄取减少，表明鼻孔闭塞确实会影响该结构。
