Wong C S, Gamble J R, Skinner M P, Lucas C M, Berndt M C, Vadas M A
Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2397-401. doi: 10.1073/pnas.88.6.2397.
The respiratory burst of blood neutrophils has a critical role in the destruction of microorganisms and tissue damage in inflammation. Neutrophils adhere in a dose-dependent fashion to granule membrane protein 140 (GMP140), a member of the LEC-CAM (lectin/epidermal growth factor/complement-binding domain cell adhesion molecule) family of adhesion proteins when it is immobilized onto plastic surfaces. Adherence to GMP140 was associated with less superoxide anion generation than adherence to other surfaces, an effect that is especially remarkable after activation of neutrophils with tumor necrosis factor alpha, an agent that on other surfaces promotes adhesion and spreading. However, on GMP140 the cells fail to spread and instead remain rounded and refractile. Neutrophils adhering to GMP140 were also deficient in superoxide anion generation to formylmethionylleucylphenylalanine. Furthermore, fluid-phase GMP140 also inhibited the superoxide generation by neutrophils stimulated by tumor necrosis factor alpha. The effect of GMP140 was reversible by washing and was inhibited by anti-GMP140 Fab antibody. GMP140 appears to be a natural antiinflammatory molecule that may prevent the inappropriate activation of neutrophils in the circulation.
血液中性粒细胞的呼吸爆发在炎症中微生物的破坏和组织损伤方面起着关键作用。当颗粒膜蛋白140（GMP140）固定在塑料表面时，中性粒细胞以剂量依赖的方式黏附于它，GMP140是凝集素/表皮生长因子/补体结合域细胞黏附分子（LEC-CAM）家族黏附蛋白的一员。与黏附于其他表面相比，黏附于GMP140时超氧阴离子的生成较少，在用肿瘤坏死因子α激活中性粒细胞后这种效应尤为显著，肿瘤坏死因子α在其他表面可促进黏附和铺展。然而，在GMP140上细胞无法铺展，而是保持圆形且有折光性。黏附于GMP140的中性粒细胞在对甲酰甲硫氨酰亮氨酰苯丙氨酸产生超氧阴离子方面也存在缺陷。此外，液相GMP140也抑制肿瘤坏死因子α刺激的中性粒细胞产生超氧阴离子。GMP140的作用可通过洗涤逆转，并被抗GMP140 Fab抗体抑制。GMP140似乎是一种天然的抗炎分子，可能会防止循环中的中性粒细胞过度激活。
