Neppelberg Evelyn, Johannessen Anne Christine
Institute of Oral Sciences, Oral Pathology and Forensic Odontology, University of Bergen, Bergen, Norway.
Eur Arch Otorhinolaryngol. 2007 Oct;264(10):1223-30. doi: 10.1007/s00405-007-0346-5. Epub 2007 May 25.
Oral lichen planus (OLP) may be associated with a small risk of malignant transformation of the oral mucosa. Using cases which had transformed, and those which had not, this study aimed to evaluate the potential of DNA content, expression of Cyclooxygenase-2 (Cox-2) and of epithelial (E)-cadherin as risk markers in lesions of OLP. We investigated 78 archival biopsies from; (1) 26 OLP patients with at least two biopsies, of whom seven presented OLP with epithelial dysplasia, followed by oral squamous cell carcinoma (OSCC) in five of them, (2) 19 OLP patients with one biopsy taken. Image cytometry for measurement of DNA content and immunohistochemistry for visualisation of Cox-2 and E-cadherin expression were performed. All OLP biopsies investigated were classified as diploid, one OLP with epithelial dysplasia was tetraploid and all OSCC were diploid. Cox-2 was detected in the epithelium of all OLP specimens investigated, as well as in epithelial dysplasias and OSCC. Focal loss of E-cadherin expression was observed in basal keratinocytes in 88% of the OLP specimens investigated, in all epithelial dysplasias and OSCC. In conclusion, neither aneuploidy, Cox-2 expression, nor loss of E-cadherin expression, were significant reliable markers to select OLP lesions at risk for development of OSCC in the present patient material.
口腔扁平苔藓（OLP）可能与口腔黏膜恶性转化的低风险相关。本研究利用已发生转化和未发生转化的病例，旨在评估DNA含量、环氧化酶-2（Cox-2）和上皮（E）-钙黏蛋白的表达作为OLP病变风险标志物的潜力。我们研究了78份存档活检样本，这些样本来自：（1）26例至少有两次活检的OLP患者，其中7例出现伴有上皮发育异常的OLP，随后5例发展为口腔鳞状细胞癌（OSCC）；（2）19例进行了一次活检的OLP患者。进行了用于测量DNA含量的图像细胞术以及用于观察Cox-2和E-钙黏蛋白表达的免疫组织化学。所有研究的OLP活检样本均分类为二倍体，1例伴有上皮发育异常的OLP为四倍体，所有OSCC均为二倍体。在所有研究的OLP标本的上皮中以及上皮发育异常和OSCC中均检测到Cox-2。在88%的研究OLP标本的基底角质形成细胞中、所有上皮发育异常和OSCC中均观察到E-钙黏蛋白表达的局灶性缺失。总之，在本患者材料中，非整倍体、Cox-2表达或E-钙黏蛋白表达缺失均不是选择有发展为OSCC风险的OLP病变的显著可靠标志物。
