IncB、IncK和IncZ质粒复制区域的比较分析。
Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.
作者信息
Praszkier J, Wei T, Siemering K, Pittard J
机构信息
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
出版信息
J Bacteriol. 1991 Apr;173(7):2393-7. doi: 10.1128/jb.173.7.2393-2397.1991.
Abstract
Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720. The coding sequence of the putative replication protein, RepA, of each plasmid was located. RepA of K and B plasmids were homologous, whereas RepA of Z resembled RepA1 of FII plasmid. Sequences upstream of RepA were conserved in the three I-complex plasmids. Group B and Z plasmids were incompatible.
摘要
构建了来自I复合体质粒R387（IncK）和pIE545（IncZ）的微型复制子，并将其复制区域的核苷酸序列与B质粒pMU720的进行了比较。确定了每个质粒推定的复制蛋白RepA的编码序列。K质粒和B质粒的RepA是同源的，而Z质粒的RepA与FII质粒的RepA1相似。RepA上游的序列在三种I复合体质粒中是保守的。B组和Z组质粒是不兼容的。
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