电离辐射后体外培养的胚胎软骨中溶酶体酶的剂量和时间依赖性增加。

Dose- and time-dependent increase of lysosomal enzymes in embryonic cartilage in vitro after ionizing radiation.

作者信息

Cornelissen M, de Ridder L

机构信息

Laboratory for Histology, State University of Gent, Belgium.

出版信息

Scanning Microsc. 1990 Sep;4(3):769-73; discussion 773-4.

PMID:1706891

Abstract

Radiation doses of 20, 50 or 100 Gy caused the same time related decrease for RNA and proteoglycan (PG) synthesis in embryonic cartilage in vitro (4 days culture). In this paper, participation of lysosomes in this radiation response is investigated. Therefore, we employ a cytochemical method using beta-glycerophosphate as substrate for acid phosphatase (AP) detection. Increase of AP was found 2 days after irradiation and increased during the whole culture period. The increase was more pronounced with a higher radiation dose. Stimulation of AP activity explains the observed radiation response of RNA and PG synthesis.

摘要

20、50或100戈瑞的辐射剂量导致体外培养（4天）的胚胎软骨中RNA和蛋白聚糖（PG）合成出现与时间相关的相同程度下降。在本文中，研究了溶酶体在这种辐射反应中的作用。因此，我们采用一种细胞化学方法，以β-甘油磷酸作为酸性磷酸酶（AP）检测的底物。照射后2天发现AP增加，并在整个培养期间持续增加。辐射剂量越高，增加越明显。AP活性的刺激解释了观察到的RNA和PG合成的辐射反应。