Osman N, Adawi D, Ahrné S, Jeppsson B, Molin G
Department of Food Technology, Engineering and Nutrition, Lund University, Lund, Sweden.
Dig Liver Dis. 2007 Sep;39(9):849-56. doi: 10.1016/j.dld.2007.06.001. Epub 2007 Jul 25.
D-galactosamine together with lipopolysaccharide can lead to a pronounced secretion by Kupffer cells of pro-inflammatory mediators, which have been shown to be early and important mediators of liver injury. Probiotics and dietary supplementation with fruit or vegetable extracts with high content of antioxidants, such as blueberry, could be beneficial in protecting against hepatotoxicity.
To investigate whether blueberry and probiotics could attenuate liver injury induced by D-galactosamine and lipopolysaccharide.
Sprague-Dawley rats were used.
Six experimental groups: acute liver injury control and five groups of liver injury treated by blueberry alone or by each of the probiotics strains (Lactobacillus plantarum DSM 15313 and Bifidobacterium infantis DSM 15159) with and without blueberry. Samples were collected 24 h after induction for bacterial test, liver function test, short chain fatty acids, myeloperoxidase, cytokines, malondialdehyde and glutathione.
Alanine aminotransferase levels decreased significantly in all groups compared to liver injury control and DSM 15313 groups. Bilirubin, liver TNF-alpha, myeloperoxidase and acetic acid in cecum content decreased significantly in all groups, while liver glutathione values increased significantly in all groups compared to liver injury control. Liver IL-1beta and bacterial translocation to the liver and mesenteric lymph nodes decreased significantly in all groups except B. infantis DSM 15159 group compared to the liver injury control. Enterobacteriaceae count in cecum decreased significantly in the groups with blueberry plus probiotics compared to the other groups.
Blueberry and probiotics exert protective effects on acute liver injury. They reduce the hepatocytes injury, the inflammation and the pro-inflammatory cytokines, and improve the barrier functions and antioxidant activity.
D-半乳糖胺与脂多糖共同作用可导致库普弗细胞大量分泌促炎介质，这些介质已被证明是肝损伤的早期重要介质。益生菌以及补充富含抗氧化剂的水果或蔬菜提取物（如蓝莓）的饮食可能有助于预防肝毒性。
研究蓝莓和益生菌是否能减轻D-半乳糖胺和脂多糖诱导的肝损伤。
使用斯普拉格-道利大鼠。
六个实验组：急性肝损伤对照组和五组肝损伤组，分别单独用蓝莓或每种益生菌菌株（植物乳杆菌DSM 15313和婴儿双歧杆菌DSM 15159）处理，其中一组同时使用蓝莓和益生菌。诱导后24小时采集样本进行细菌检测、肝功能检测、短链脂肪酸检测、髓过氧化物酶检测、细胞因子检测、丙二醛检测和谷胱甘肽检测。
与肝损伤对照组和DSM 15313组相比，所有组的丙氨酸转氨酶水平均显著降低。所有组的胆红素、肝脏肿瘤坏死因子-α、髓过氧化物酶和盲肠内容物中的乙酸均显著降低，而与肝损伤对照组相比，所有组的肝脏谷胱甘肽值均显著升高。与肝损伤对照组相比，除婴儿双歧杆菌DSM 15159组外，所有组的肝脏白细胞介素-1β以及细菌向肝脏和肠系膜淋巴结的移位均显著降低。与其他组相比，蓝莓加益生菌组的盲肠肠杆菌科计数显著降低。
蓝莓和益生菌对急性肝损伤具有保护作用。它们可减轻肝细胞损伤、炎症和促炎细胞因子，改善屏障功能和抗氧化活性。
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