Zhang Zhan-Jun, Li Peng, Wang Zhong, Li Peng-Tao, Zhang Wen-Sheng, Sun Zhi-Hua, Zhang Xue-Jun, Wang Yong-Yan
Beijing Normal University, The Key Laboratory of Traditional Chinese Medicine Protection and Utilization, 19 XinJieKouWai Street, HaiDian District, Beijing, PA 100875, China.
Brain Res. 2006 Dec 6;1123(1):188-95. doi: 10.1016/j.brainres.2006.09.063. Epub 2006 Oct 27.
To compare the individual effects of baicalin and jasminoidin with the combined effect of them on cerebral ischemia-reperfusion injury, and test whether the combined administration of baicalin and jasminoidin can improve the therapeutic effect. Male Sprague-Dawley rats underwent focal cerebral ischemia for 1.5 h and reperfusion for 24 h. Just before reperfusion, tested drugs (baicalin, jasminoidin, a drug combination consisting of baicalin and jasminoidin, or nimodipine) were intravenously treated. Diffusion weighted imaging (DWI) of magnetic resonance imaging (MRI), behavior examination, 2,3,5-triphenyltetrazolium chloride (TTC) staining, histological examination, and real-time PCR for BDNF and caspase-3 were performed. All of the drug treatments could significantly ameliorate the results of TTC and histological examination, and the baicalin/jasminoidin combination did so most prominently. This combination could also significantly ameliorate DWI of MRI and behavior examination results, and promote the expression of BDNF and inhibit the expression of caspase-3. On the whole, both baicalin and jasminoidin have a preventive effect against ischemic stroke, although their effects are not very strong. However, the combination of baicalin and jasminoidin can significantly improve their effectiveness. This may be related to its better regulation on the BDNF and caspase-3.
为比较黄芩苷和栀子苷单独作用与二者联合作用对脑缺血再灌注损伤的影响，并检测黄芩苷与栀子苷联合给药是否能提高治疗效果。雄性Sprague-Dawley大鼠进行局灶性脑缺血1.5小时并再灌注24小时。在再灌注前，对受试药物（黄芩苷、栀子苷、由黄芩苷和栀子苷组成的药物组合或尼莫地平）进行静脉给药。进行磁共振成像（MRI）的扩散加权成像（DWI）、行为检查、2,3,5-三苯基四氮唑氯化物（TTC）染色、组织学检查以及脑源性神经营养因子（BDNF）和半胱天冬酶-3（caspase-3）的实时聚合酶链反应（PCR）。所有药物治疗均能显著改善TTC和组织学检查结果，其中黄芩苷/栀子苷组合最为显著。该组合还能显著改善MRI的DWI和行为检查结果，并促进BDNF的表达和抑制caspase-3的表达。总体而言，黄芩苷和栀子苷对缺血性中风均有预防作用，尽管其作用不是很强。然而，黄芩苷与栀子苷的组合可显著提高其有效性。这可能与其对BDNF和caspase-3的更好调节有关。
