Linares Victoria, Bellés Montserrat, Albina M Luisa, Sirvent Juan J, Sánchez Domènec J, Domingo José L
Laboratory of Toxicology and Environmental Health, School of Medicine, Rovira i Virgili University, San Lorenzo 21, 43201 Reus, Spain.
Toxicol Lett. 2006 Dec 1;167(2):152-61. doi: 10.1016/j.toxlet.2006.09.004. Epub 2006 Sep 17.
The pro-oxidant activity of uranium (U) was assessed in kidney and testes of male rats, tissues in which toxic effects of this metal are well established. Eight groups of Sprague-Dawley rats received uranyl acetate dihydrate (UAD) in the drinking water at 0, 10, 20, and 40 mg/kgday for 3 months. Rats in four groups were concurrently subjected to restraint during 2 h/day throughout the study. Histopathological examination of the kidneys revealed an angiomatose transformation in U-treated animals. In kidney, thiobarbituric acid-reactive substances (TBARS) levels and oxidized glutathione (GSSG) activity were correlated with U exposure. The superoxide dismutase (SOD) activity was significantly enhanced in both kidney and testis. Oral UAD administration induced a decrease of glutathione reductase (GR) and reduced glutathione (GSH) in the male reproductive tract. The results of this study suggest that graded doses of U elicit depletion of the antioxidant defence system of the rat and induce oxidative stress in testes and kidneys. Although at the current U doses, restraint stress scarcely showed additional adverse effects, its potential influence should not be underrated.
在雄性大鼠的肾脏和睾丸中评估了铀（U）的促氧化活性，这两种组织中该金属的毒性作用已得到充分证实。八组斯普拉格-道利大鼠在饮用水中分别接受0、10、20和40mg/kg·天的二水合醋酸双氧铀（UAD），持续3个月。在整个研究过程中，四组大鼠每天同时接受2小时的束缚。对肾脏的组织病理学检查显示，接受铀处理的动物出现血管瘤样转变。在肾脏中，硫代巴比妥酸反应性物质（TBARS）水平和氧化型谷胱甘肽（GSSG）活性与铀暴露相关。超氧化物歧化酶（SOD）活性在肾脏和睾丸中均显著增强。口服UAD导致雄性生殖道中谷胱甘肽还原酶（GR）和还原型谷胱甘肽（GSH）减少。本研究结果表明，分级剂量的铀会导致大鼠抗氧化防御系统耗竭，并在睾丸和肾脏中诱导氧化应激。尽管在当前的铀剂量下，束缚应激几乎没有显示出额外的不良影响，但其潜在影响不应被低估。
