Kleschyov Andrei L, Wenzel Philip, Munzel Thomas
Second Department of Medicine, Johannes Gutenberg University of Mainz, Mainz 55131, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 15;851(1-2):12-20. doi: 10.1016/j.jchromb.2006.10.006. Epub 2006 Oct 27.
Nitric oxide (NO) is a free radical species with multiple physiological functions. Because of low concentrations and short half-life of NO, its direct measurement in living tissues remains a difficult task. Electron paramagnetic resonance (EPR) spin trapping is probably one of the best suitable platforms for development of new methods for quantification of biological NO. The most reliable EPR-based approaches developed so far are based on the reaction of NO with various iron complexes, both intrinsic and exogenously applied. This review is focused on the current state and perspectives of EPR spin trapping for experimental and clinical NO biology.
一氧化氮（NO）是一种具有多种生理功能的自由基。由于NO在生物组织中的浓度较低且半衰期较短，因此在活体组织中直接测量NO仍然是一项艰巨的任务。电子顺磁共振（EPR）自旋捕获技术可能是开发定量生物NO新方法的最合适平台之一。目前基于EPR开发的最可靠方法是基于NO与各种铁配合物的反应，包括内源性和外源性应用的铁配合物。这篇综述聚焦于EPR自旋捕获技术在实验和临床NO生物学中的现状与前景。
