Israr Mohd, Sahi Shivendra V
Department of Biology, Western Kentucky University, 1906 College Heights Blvd. #11080, Bowling Green, KY 42101-1080, USA.
Plant Physiol Biochem. 2006 Oct;44(10):590-5. doi: 10.1016/j.plaphy.2006.09.021. Epub 2006 Oct 27.
The effect of mercury (Hg) on the growth and the response of antioxidative systems have been investigated in Sesbania cell cultures to determine the tolerance limits and the mechanisms of metal (Hg) tolerance in plant cells. Cell cultures of Sesbania were developed in different concentrations (0-50 microM) of mercury. Cultures tolerated Hg up to a concentration of 40 microM and showed an increase in the fresh weight growth by 620% in 3 weeks. The levels of antioxidants: glutathione (GSH) and non-protein thiols (NPSH) and the activities of antioxidative enzymes: superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) were influenced by Hg treatments. The contents of GSH, NPSH and GSH/GSSG ratio increased up to a concentration of 40 muM Hg and then severely declined at 50 microM Hg. The activities of antioxidative enzymes, SOD, APX and GR followed the same trends as antioxidants, first increased up to a concentration of 40 muM Hg and then declined in the presence of 50 microM Hg.
为了确定植物细胞对汞（Hg）的耐受极限和耐受机制，研究了汞对田菁细胞培养物生长及抗氧化系统反应的影响。在不同浓度（0 - 50微摩尔）的汞中培养田菁细胞。培养物对高达40微摩尔浓度的汞具有耐受性，并且在3周内鲜重增长增加了620%。抗氧化剂谷胱甘肽（GSH）和非蛋白硫醇（NPSH）的水平以及抗氧化酶超氧化物歧化酶（SOD，EC 1.15.1.1）、抗坏血酸过氧化物酶（APX，EC 1.11.1.11）和谷胱甘肽还原酶（GR，EC 1.6.4.2）的活性受汞处理的影响。GSH、NPSH的含量以及GSH/GSSG比值在汞浓度达到40微摩尔时增加，然后在50微摩尔汞时急剧下降。抗氧化酶SOD、APX和GR的活性与抗氧化剂遵循相同的趋势，首先在汞浓度达到40微摩尔时增加，然后在50微摩尔汞存在时下降。
