Eliaspour Siamak, Seyed Sharifi Raouf, Shirkhani Ali, Farzaneh Salim
Department of Agronomy and Plant Breeding Faculty of Agriculture and Natural Resources University of Mohaghegh Ardabili Ardabil Iran.
Crops and Horticulture Research Department Kermanshah Agricultural Resources Research and Education Center (AREEO) Kermanshah Iran.
Food Sci Nutr. 2020 Sep 25;8(11):5985-5998. doi: 10.1002/fsn3.1884. eCollection 2020 Nov.
In this research, effects iron nano-oxide and biofertilizers and chemical was investigated on the yield and some traits of Maize under normal and drought stress conditions in two years (2018 and 2019). The experiment was performed in the form of split-spilt plot in a complete random block design with three replications. The studied irrigation treatment included three levels (normal, 85% and 65% optimum water requirement) in the main plots and iron nano-oxide at four levels (0, 0.5, 1, and 1.5 g/L) in subplots, and biofertilizers at four levels (noninoculation, inoculation with mycorrhiza, inoculation with pseudomonas and combined inoculation of mycorrhiza and pseudomonas) in sub-plots. The results showed that grain yield, 1000-grain weight, and leaf chlorophyll contents decreased by drought stress. Use of pseudomonas and mycorrhiza increased these traits in normal and stress conditions, but iron nano-oxide had no significant effect on the measured traits. Also, drought stress increased malondialdehyde, ion leakage, catalase, peroxidase, proline, and polyphenol oxidase in both light and severe stress regimes. The amount of antioxidant enzymes increased under drought stress conditions in corn. The results indicated that all the characteristics measured by double inoculation with Pseudomonas and Microoriza had the best performance in conditions of water shortage and the use of these biofertilizers increases yield, 1000-seed weight, and chlorophyll content of maize. Also, the use of biofertilizers modulates the effect of drought stress and reduces its negative effects.
本研究在2018年和2019年两年间，研究了纳米氧化铁、生物肥料和化学肥料对正常和干旱胁迫条件下玉米产量及一些性状的影响。试验采用裂区裂区设计，完全随机区组排列，重复3次。研究的灌溉处理包括主区的三个水平（正常、85%和65%最佳需水量），副区的纳米氧化铁四个水平（0、0.5、1和1.5 g/L），以及副区的生物肥料四个水平（不接种、接种菌根、接种假单胞菌以及菌根和假单胞菌联合接种）。结果表明，干旱胁迫使玉米籽粒产量、千粒重和叶片叶绿素含量降低。在正常和胁迫条件下，接种假单胞菌和菌根可提高这些性状，但纳米氧化铁对所测性状无显著影响。此外，在轻度和重度胁迫条件下，干旱胁迫均增加了丙二醛、离子渗漏、过氧化氢酶、过氧化物酶、脯氨酸和多酚氧化酶的含量。干旱胁迫条件下玉米抗氧化酶含量增加。结果表明，在水分短缺条件下，假单胞菌和菌根双接种所测的所有性状表现最佳，使用这些生物肥料可提高玉米产量、千粒重和叶绿素含量。此外，生物肥料的使用可调节干旱胁迫的影响并降低其负面影响。
