Doudna J A, Couture S, Szostak J W
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Science. 1991 Mar 29;251(5001):1605-8. doi: 10.1126/science.1707185.
Derivatives of the sunY self-splicing intron efficiently catalyzed the synthesis of complementary strand RNA by template-directed assembly of oligonucleotides. These ribozymes were separated into three short RNA fragments that formed active catalytic complexes. One of the multisubunit sunY derivatives catalyzed the synthesis of a strand of RNA complementary to one of its own subunits. These results suggest that prebiotically synthesized oligonucleotides might have been able to assemble into a complex capable of self-replication.
sunY自剪接内含子的衍生物通过寡核苷酸的模板导向组装有效地催化了互补链RNA的合成。这些核酶被分离成三个短RNA片段，它们形成了活性催化复合物。一种多亚基sunY衍生物催化合成了与其自身一个亚基互补的RNA链。这些结果表明，益生元合成的寡核苷酸可能能够组装成一个能够自我复制的复合物。
