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由嗜热四膜虫核酶催化的模板导向引物延伸反应。

Template-directed primer extension catalyzed by the Tetrahymena ribozyme.

作者信息

Bartel D P, Doudna J A, Usman N, Szostak J W

机构信息

Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.

出版信息

Mol Cell Biol. 1991 Jun;11(6):3390-4. doi: 10.1128/mcb.11.6.3390-3394.1991.

DOI:10.1128/mcb.11.6.3390-3394.1991
PMID:2038341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360195/
Abstract

The Tetrahymena ribozyme has been shown to catalyze an RNA polymerase-like reaction in which an RNA primer is extended by the sequential addition of pN nucleotides derived from GpN dinucleotides, where N = A, C, or U. Here, we show that this reaction is influenced by the presence of a template; bases that can form Watson-Crick base pairs with a template add as much as 25-fold more efficiently than mismatched bases. A mutant enzyme with an altered guanosine binding site can catalyze template-directed primer extension with all four bases when supplied with dinucleotides of the form 2-aminopurine-pN.

摘要

嗜热四膜虫核酶已被证明能催化一种类似RNA聚合酶的反应，即RNA引物通过依次添加源自GpN二核苷酸的pN核苷酸得以延伸，其中N = A、C或U。在此，我们表明该反应受模板存在的影响；能与模板形成沃森-克里克碱基对的碱基添加效率比错配碱基高多达25倍。当提供2-氨基嘌呤-pN形式的二核苷酸时，一种鸟苷结合位点发生改变的突变酶能够催化以所有四种碱基为模板的引物延伸反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61a1/360195/7fca4d6b46d7/molcellb00140-0485-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61a1/360195/42864701ddae/molcellb00140-0483-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61a1/360195/7fca4d6b46d7/molcellb00140-0485-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61a1/360195/42864701ddae/molcellb00140-0483-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61a1/360195/7fca4d6b46d7/molcellb00140-0485-a.jpg

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