柞蚕核型多角体病毒p11基因的特性研究，该基因是苜蓿银纹夜蛾核型多角体病毒orf108的同源物。

Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108.

作者信息

Shi Sheng-Lin, Pan Min-Hui, Lu Cheng

机构信息

Key Sericultural Laboratory of Agriculture Ministry, College of Sericulture and Biotechnology, Southwest University, Chongqing, 400716, China.

出版信息

Virus Genes. 2007 Aug;35(1):97-101. doi: 10.1007/s11262-006-0033-4. Epub 2006 Oct 27.

DOI:10.1007/s11262-006-0033-4

PMID:17072759

Abstract

Antheraea pernyi nucleopolyhedrovirus (ApNPV) p11 gene is 309 bp long, potentially encoding 102 amino acids with a predicted molecular weight of 11.2 kDa. ApNPV p11 gene was cloned into the prokaryotic expression vector pQE-30 and P11 was expressed in E. coli M15. Polyclonal antiserum was made against 6xHis tagged P11 protein expressed in E. coli M15. P11 gene transcription was detected as early as 36 h post-infection (p.i.) in tussah pupa and remain at high level up to 96 h p.i. Structural localization revealed that P11 protein was present in polyhedral inclusion bodies (PIB) dilute alkaline saline (DAS) pellet (P) fractions and occlusion-derived virus (ODV), but not in PIB DAS supernatant (S) fractions and budded virus (BV). These results indicated that P11 was associated with ApNPV structure.

摘要

柞蚕核型多角体病毒（ApNPV）的p11基因长度为309 bp，可能编码102个氨基酸，预测分子量为11.2 kDa。将ApNPV p11基因克隆到原核表达载体pQE - 30中，并在大肠杆菌M15中表达P11。针对在大肠杆菌M15中表达的6xHis标记的P11蛋白制备了多克隆抗血清。在柞蚕蛹感染后36小时（p.i.）最早检测到P11基因转录，并且在感染后96小时一直保持在高水平。结构定位显示，P11蛋白存在于多角体包涵体（PIB）稀碱性盐水（DAS）沉淀（P）组分和包涵体衍生病毒（ODV）中，但不存在于PIB DAS上清液（S）组分和出芽病毒（BV）中。这些结果表明P11与ApNPV结构相关。