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新松叶蜂核型多角体病毒基因组的序列分析

Sequence analysis of the genome of the Neodiprion sertifer nucleopolyhedrovirus.

作者信息

Garcia-Maruniak Alejandra, Maruniak James E, Zanotto Paolo M A, Doumbouya Aissa E, Liu Jaw-Ching, Merritt Thomas M, Lanoie Jennifer S

机构信息

Entomology and Nematology Department, University of Florida, Gainesville, FL 32611-0620, USA.

出版信息

J Virol. 2004 Jul;78(13):7036-51. doi: 10.1128/JVI.78.13.7036-7051.2004.

Abstract

The genome of the Neodiprion sertifer nucleopolyhedrovirus (NeseNPV), which infects the European pine sawfly, N. sertifer (Hymenoptera: Diprionidae), was sequenced and analyzed. The genome was 86,462 bp in size. The C+G content of 34% was lower than that of the majority of baculoviruses. A total of 90 methionine-initiated open reading frames (ORFs) with more than 50 amino acids and minimal overlapping were found. From those, 43 ORFs were homologous to other baculovirus ORFs, and 29 of these were from the 30 conserved core genes among all baculoviruses. A NeseNPV homolog to the ld130 gene, which is present in all other baculovirus genomes sequenced to date, could not be identified. Six NeseNPV ORFs were similar to non-baculovirus-related genes, one of which was a trypsin-like gene. Only one iap gene, containing a single BIR motif and a RING finger, was found in NeseNPV. Two NeseNPV ORFs (nese18 and nese19) were duplicates transcribed in opposite orientations from each other. NeseNPV did not have an AcMNPV ORF 2 homolog characterized as the baculovirus repeat ORF (bro). Six homologous regions (hrs) were located within the NeseNPV genome, each containing small palindromes embedded within direct repeats. A phylogenetic analysis was done to root the tree based upon the sequences of DNA polymerase genes of NeseNPV, 23 other baculoviruses, and other phyla. Baculovirus phylogeny was then constructed with 29 conserved genes from 24 baculovirus genomes. Culex nigripalpus nucleopolyhedrovirus (CuniNPV) was the most distantly related baculovirus, branching to the hymenopteran NeseNPV and the lepidopteran nucleopolyhedroviruses and granuloviruses.

摘要

感染欧洲松叶蜂(Neodiprion sertifer,膜翅目:松叶蜂科)的新松叶蜂核型多角体病毒(NeseNPV)的基因组已被测序和分析。该基因组大小为86,462 bp。34%的C+G含量低于大多数杆状病毒。共发现90个由甲硫氨酸起始、含有50个以上氨基酸且重叠最少的开放阅读框(ORF)。其中,43个ORF与其他杆状病毒的ORF同源,其中29个来自所有杆状病毒中的30个保守核心基因。未鉴定出与迄今已测序的所有其他杆状病毒基因组中都存在的ld130基因同源的NeseNPV基因。6个NeseNPV ORF与非杆状病毒相关基因相似,其中一个是类胰蛋白酶基因。在NeseNPV中仅发现一个iap基因,其含有一个单一的杆状病毒IAP重复序列(BIR)基序和一个锌指结构。两个NeseNPV ORF(nese18和nese19)是彼此反向转录的重复序列。NeseNPV没有被鉴定为杆状病毒重复ORF(bro)的AcMNPV ORF 2同源物。在NeseNPV基因组内定位了6个同源区域(hr),每个区域都包含嵌入在正向重复序列中的小回文序列。基于NeseNPV、其他23种杆状病毒以及其他门类的DNA聚合酶基因序列进行了系统发育分析以确定树的根。然后用来自24种杆状病毒基因组的29个保守基因构建了杆状病毒系统发育树。致倦库蚊核型多角体病毒(CuniNPV)是亲缘关系最远的杆状病毒,分支到膜翅目的NeseNPV以及鳞翅目的核型多角体病毒和颗粒体病毒。

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