Yadav J S, Doddapaneni H, Subramanian V
Molecular Toxicology Division, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0056, USA.
Biochem Soc Trans. 2006 Dec;34(Pt 6):1165-9. doi: 10.1042/BST0341165.
The model white rot fungus Phanerochaete chrysosporium has the extraordinary ability to degrade (to CO(2)) lignin and detoxify a variety of chemical pollutants. Whole genome sequencing of this fungus has revealed the presence of the largest P450ome in fungi comprising approx. 150 P450 genes, most of which have unknown function. On the basis of our genome-wide structural and evolutionary analysis, these P450 genes could be classified into 12 families and 23 subfamilies and under 11 fungal P450 clans. The analysis further revealed an extensive gene clustering with a total of 16 P450 clusters constituted of up to 11 members per cluster. In particular, evidence and role of gene duplications and horizontal gene transfer in the evolution of these P450 clusters have been discussed using two of the P450 families [CYP63 and CYP505 (where CYP is cytochrome P450)] as examples. In addition, the observed differential transcriptional induction of the clustered members of the CYP63 gene family, in response to different xenobiotic chemicals and carbon sources, indicated functional divergence within the P450 clusters, of this basidiomycete fungus.
典型的白腐真菌黄孢原毛平革菌具有将木质素降解为二氧化碳以及使多种化学污染物解毒的非凡能力。对该真菌进行的全基因组测序显示，其拥有真菌中最大的细胞色素P450基因家族，约含150个P450基因，其中大部分基因功能未知。基于我们的全基因组结构和进化分析，这些P450基因可分为12个家族和23个亚家族，隶属于11个真菌P450族。分析还揭示了广泛的基因成簇现象，共有16个P450基因簇，每个基因簇最多由11个成员组成。特别是，以两个P450家族[CYP63和CYP505（其中CYP为细胞色素P450）]为例，讨论了基因重复和水平基因转移在这些P450基因簇进化过程中的证据及作用。此外，观察到CYP63基因家族成簇成员对不同的外源化学物质和碳源有不同的转录诱导反应，这表明该担子菌真菌的P450基因簇内存在功能分化。
