Boekhorst Jos, Wels Michiel, Kleerebezem Michiel, Siezen Roland J
Center for Molecular and Biomolecular Informatics, Radboud University Nijmegen Medical Centre, 6500HB Nijmegen, The Netherlands.
Wageningen Centre for Food Sciences, Wageningen, The Netherlands.
Microbiology (Reading). 2006 Nov;152(Pt 11):3175-3183. doi: 10.1099/mic.0.29217-0.
The predicted extracellular proteins of the bacterium Lactobacillus plantarum were analysed to gain insight into the mechanisms underlying interactions of this bacterium with its environment. Extracellular proteins play important roles in processes ranging from probiotic effects in the gastrointestinal tract to degradation of complex extracellular carbon sources such as those found in plant materials, and they have a primary role in the adaptation of a bacterium to changing environmental conditions. The functional annotation of extracellular proteins was improved using a wide variety of bioinformatics methods, including domain analysis and phylogenetic profiling. At least 12 proteins are predicted to be directly involved in adherence to host components such as collagen and mucin, and about 30 extracellular enzymes, mainly hydrolases and transglycosylases, might play a role in the degradation of substrates by L. plantarum to sustain its growth in different environmental niches. A comprehensive overview of all predicted extracellular proteins, their domains composition and their predicted function is provided through a database at http://www.cmbi.ru.nl/secretome which could serve as a basis for targeted experimental studies into the function of extracellular proteins.
对植物乳杆菌的预测胞外蛋白进行了分析，以深入了解该细菌与其环境相互作用的潜在机制。胞外蛋白在从胃肠道中的益生菌作用到复杂胞外碳源（如植物材料中发现的碳源）降解等过程中发挥着重要作用，并且在细菌适应不断变化的环境条件方面起着主要作用。使用包括结构域分析和系统发育谱分析在内的多种生物信息学方法改进了胞外蛋白的功能注释。预计至少有12种蛋白质直接参与对宿主成分（如胶原蛋白和粘蛋白）的粘附，约30种胞外酶（主要是水解酶和转糖基酶）可能在植物乳杆菌降解底物以维持其在不同环境生态位中的生长中发挥作用。通过http://www.cmbi.ru.nl/secretome网站上的数据库提供了所有预测胞外蛋白、其结构域组成及其预测功能的全面概述，该数据库可作为针对胞外蛋白功能进行靶向实验研究的基础。
