Roach T I, Else K J, Wakelin D, McLaren D J, Grencis R K
Department of Zoology, University of Nottingham.
Parasite Immunol. 1991 Jan;13(1):1-12. doi: 10.1111/j.1365-3024.1991.tb00258.x.
Mesenteric node lymphocytes from mice that had been infected with the nematode Trichuris muris, and then boosted with adult worm excretory-secretory antigens were fused with myeloma cells to produce a panel of 9 monoclonal antibodies (MoAbs). Five of the MoAbs were of the IgA isotype. The antigen recognition profiles of these MoAbs were studied using SDS-PAGE and immunoblotting; three major profile patterns were identified. Five MoAbs recognized a major band in the MW range 43-48 kD; all recognized a range of antigens. Three MoAbs were used to localize antigens in the bodies of adult worms. Granules within the anterior stichocytes were recognized strongly, as was material within the eggs and pseudocoelom. Two MoAbs stained the cuticle. Although the phosphorylcholine (PC) determinant was widely distributed within worm tissues none of the MoAbs tested recognized PC. Passive transfer of immunity was achieved using two of the IgA monoclonals; no immunity was transferred by the IgM and IgG MoAbs used. The limited recognition profiles of these IgA MoAbs, and the ability to stain stichocyte granules, suggest that their protective activity results from an interaction with ES antigens.
从感染了线虫鞭虫的小鼠肠系膜淋巴结中分离淋巴细胞，随后用成虫排泄分泌抗原进行加强免疫，之后将这些淋巴细胞与骨髓瘤细胞融合，产生了一组9种单克隆抗体（MoAbs）。其中5种MoAbs为IgA同种型。利用SDS - PAGE和免疫印迹研究了这些MoAbs的抗原识别谱；确定了三种主要的谱型。5种MoAbs识别出一条分子量在43 - 48 kD范围内的主要条带；所有这些MoAbs都识别一系列抗原。使用3种MoAbs将抗原定位在成虫体内。前刺细胞内的颗粒被强烈识别，卵和假体腔内的物质也是如此。2种MoAbs对表皮进行了染色。尽管磷酰胆碱（PC）决定簇广泛分布于虫体组织内，但所测试的MoAbs均未识别出PC。使用2种IgA单克隆抗体实现了免疫的被动转移；所使用的IgM和IgG MoAbs未转移免疫。这些IgA MoAbs有限的识别谱以及对刺细胞颗粒染色的能力表明，它们的保护活性源于与排泄分泌抗原的相互作用。
