Cadwallader Adam B, Yost H Joseph
Huntsman Cancer Institute, Center for Children, Department of Oncological Sciences, University of Utah, Salt Lake City, Utah 84112, USA.
Dev Dyn. 2006 Dec;235(12):3432-7. doi: 10.1002/dvdy.20990.
Heparan sulfate (HS) is an unbranched chain of repetitive disaccharides, which specifically binds ligands when attached to the cell surface or secreted extracellularly. HS chains contain sulfated domains termed the HS fine structure, which gives HS specific binding affinities for extracellular ligands. HS 6-O-sulfotransferases (6-OST) catalyze the transfer of sulfate groups to the 6-O position of glucosamine residues of HS. We report here the characterization and developmental expression analysis of the 6-OST gene family in the zebrafish. The zebrafish 6-OST gene family consists of four conserved vertebrate orthologues, including a gene duplication specific to zebrafish. We examined the mRNA expression patterns in several tissues/organs throughout early zebrafish development, including early cleavage stages, eyes, somites, brain, internal organ primordial, and pectoral fin development. Members of the 6-OST gene family have spatially and temporally distinct restricted expression, suggesting in vivo functional differences exist between members of this family.
硫酸乙酰肝素（HS）是由重复二糖组成的无分支链，当附着于细胞表面或分泌到细胞外时，它能特异性结合配体。HS链含有被称为HS精细结构的硫酸化结构域，这赋予了HS对细胞外配体的特异性结合亲和力。HS 6-O-硫酸转移酶（6-OST）催化硫酸基团转移到HS的葡糖胺残基的6-O位置。我们在此报告斑马鱼中6-OST基因家族的特征及发育表达分析。斑马鱼6-OST基因家族由四个保守的脊椎动物直系同源基因组成，包括一个斑马鱼特有的基因复制。我们研究了斑马鱼早期发育过程中多个组织/器官的mRNA表达模式，包括早期卵裂阶段、眼睛、体节、脑、内脏原基和胸鳍发育。6-OST基因家族成员具有空间和时间上不同的受限表达，表明该家族成员之间存在体内功能差异。
