Cobell Will, Duflo Suzy M, Magrufov Akhmar, Thibeault Susan L
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, School of Medicine, University of Utah, Salt Lake City, USA.
Ann Otol Rhinol Laryngol. 2006 Oct;115(10):764-8. doi: 10.1177/000348940611501009.
The development of a minimally invasive fine needle aspiration (FNA) technique for vocal fold (VF) biopsy would have far-reaching implications and applicability in laryngology. The objective of this study was to determine whether FNA of the VF lamina propria is feasible and whether it causes injury to the VF.
Unilateral VF FNA was performed with a 26-gauge needle on 20 rabbits. The FNA cell collection of the lamina propria was confirmed by cytology. Four weeks after the FNA, the rabbits were painlessly sacrificed and the larynges were harvested. Histologic analysis of the VF lamina propria included Masson's trichrome and elastin-van Gieson stains. The linear viscoelastic shear properties, elastic modulus, and viscous modulus of the tissue were measured.
Compared to the contralateral normal VFs, those VFs that underwent FNA demonstrated no significant differences in levels of collagen (p = .17) and elastin (p = .94). Rheologically, the elastic shear modulus and viscous modulus did not significantly differ between the normal and FNA VFs (p = .9380 and p = .9359, respectively).
Fine needle aspiration of the VF lamina propria is feasible without injury and offers a potentially promising, less invasive alternative to be developed for future diagnostic and therapeutic management of VF lesions.
开发一种用于声带活检的微创细针穿刺（FNA）技术在喉科学中将具有深远的意义和适用性。本研究的目的是确定对声带固有层进行细针穿刺是否可行以及是否会对声带造成损伤。
用26号针在20只兔子身上进行单侧声带细针穿刺。通过细胞学检查确认固有层的细针穿刺细胞采集情况。细针穿刺四周后，对兔子实施无痛安乐死并摘取喉部。对声带固有层进行组织学分析，包括采用马森三色染色法和弹性蛋白-范吉森染色法。测量组织的线性粘弹性剪切特性、弹性模量和粘性模量。
与对侧正常声带相比，接受细针穿刺的声带在胶原蛋白水平（p = 0.17）和弹性蛋白水平（p = 0.94）上无显著差异。从流变学角度来看，正常声带和接受细针穿刺的声带之间的弹性剪切模量和粘性模量无显著差异（分别为p = 0.9380和p = 0.9359）。
对声带固有层进行细针穿刺是可行的，且不会造成损伤，为未来声带病变的诊断和治疗管理提供了一种潜在的、有前景的、侵入性较小的替代方法。
