Kodama Haruka, Kumai Yoshihiko, Nishimoto Kohei, Toya Yutaka, Miyamaru Satoru, Furushima Shinobu, Yumoto Eiji
1 Department of Otolaryngology Head and Neck Surgery, Kumamoto University Graduate School of Medicine, Kumamoto, Japan.
2 Division of Microbiology and Genetics, Institute of Resource Development and Analysis, Kumamoto University School of Medicine, Kumamoto, Japan.
Ann Otol Rhinol Laryngol. 2018 Mar;127(3):146-154. doi: 10.1177/0003489417750165. Epub 2018 Jan 9.
To develop a vocal fold (VF) scarring procedure in the ferret, characterize the scars histologically, and test the injectability of the lamina propria (LP). Secondarily, to compare laryngeal anatomy of the ferret with rat and rabbit.
The larynges of 18 male ferrets were prepared by unilateral scarring, and normal larynges from 6 female Wistar rats and 5 male albino rabbits were used for comparative purposes. For scarring, the right VF were electrocauterized, ablating the entire LP. Prior to harvesting the larynges at 4 and 16 weeks, each ferret was re-anesthetized, and in 3 animals, India ink was injected into the LPs of both normal and scarred VFs.
Laryngoscopic methods and instrumentation for precise visualization, scarring, and injection were developed. The scarred VFs had reduced hyaluronic acid and increased collagen type I, III, and fibronectin compared with normal VFs. The 2 timepoints (4 and 16 weeks) differed significantly only in collagen type III level (levels were higher at 4 weeks). Injected ink migrated from scarred LP to muscle layer just beneath the scarred tissue 3 hours after injection.
The ferret is a promising species for creation and experimental treatment of vocal fold scar.
在雪貂身上开发一种声带瘢痕形成程序，对瘢痕进行组织学特征分析，并测试固有层（LP）的可注射性。其次，比较雪貂与大鼠和兔子的喉部解剖结构。
通过单侧瘢痕形成制备18只雄性雪貂的喉部，并使用6只雌性Wistar大鼠和5只雄性白化兔的正常喉部进行比较。为形成瘢痕，对右侧声带进行电灼，切除整个固有层。在4周和16周采集喉部之前，对每只雪貂再次进行麻醉，并对3只动物的正常和瘢痕化声带的固有层注射印度墨水。
开发了用于精确可视化、瘢痕形成和注射的喉镜检查方法及仪器。与正常声带相比，瘢痕化声带的透明质酸减少，I型、III型胶原蛋白和纤连蛋白增加。两个时间点（4周和16周）仅在III型胶原蛋白水平上有显著差异（4周时水平更高）。注射后3小时，注入的墨水从瘢痕化的固有层迁移到瘢痕化组织下方的肌肉层。
雪貂是创建和实验性治疗声带瘢痕的有前景的物种。
