Kulkarni-Kale Urmila, Bhosle Shriram, Kolaskar A S
Bioinformatics Centre, University of Pune, Pune 411 007, India.
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W168-71. doi: 10.1093/nar/gki460.
CEP server (http://bioinfo.ernet.in/cep.htm) provides a web interface to the conformational epitope prediction algorithm developed in-house. The algorithm, apart from predicting conformational epitopes, also predicts antigenic determinants and sequential epitopes. The epitopes are predicted using 3D structure data of protein antigens, which can be visualized graphically. The algorithm employs structure-based Bioinformatics approach and solvent accessibility of amino acids in an explicit manner. Accuracy of the algorithm was found to be 75% when evaluated using X-ray crystal structures of Ag-Ab complexes available in the PDB. This is the first and the only method available for the prediction of conformational epitopes, which is an attempt to map probable antibody-binding sites of protein antigens.
