Bannantine John P, Stabel Judith R, Kapur Vivek
USDA-Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA.
Department of Animal Science, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
Vaccines (Basel). 2022 Mar 26;10(4):518. doi: 10.3390/vaccines10040518.
Johne's disease affects ruminants causing an economic burden to dairy, meat and wool industries. Vaccination against subspecies (), which causes Johne's disease, is a primary intervention for disease control in livestock. Previously, a comprehensive, multi-institutional vaccine trial for Johne's disease was conducted to test the efficacy of live attenuated strains. Here, we report the humoral and cell-mediated immune responses from kid goats enrolled in that trial. Both vaccinated and unvaccinated animals showed IFN-γ stimulation and proliferation of T cell subpopulations on challenge with . CD4+, CD25+ and γδ cells from cultured PBMCs in the vaccinated goats showed significantly greater proliferation responses on stimulation with antigens. The increase in CD44+ and decrease in CD62L+ cells suggest that vaccine administration reduced the inflammatory responses associated with infection. Overall, a stronger antibody response was observed in the infected goats as compared to vaccinated goats. Two independent experimental approaches were used to identify differences in the antibody responses of vaccinated and unvaccinated goats. The first approach involved screening a phage expression library with pooled serum from infected goats, identifying previously reported antigens, including MAP_1272c and MAP_1569. However, three specific antigens detected only by vaccinated goats were also identified in the library screens. A second approach using dot blot analysis identified two additional differentially reacting proteins in the vaccinated goats (MAP_4106 and MAP_4141). These immunological results, combined with the microbiological and pathological findings obtained previously, provide a more complete picture of Johne's disease control in goats vaccinated against .
副结核（Johne's）病影响反刍动物，给乳制品、肉类和羊毛产业带来经济负担。针对引发副结核（Johne's）病的亚种（）进行疫苗接种是家畜疾病控制的主要干预措施。此前，曾开展一项针对副结核（Johne's）病的综合性多机构疫苗试验，以测试减毒活菌株的功效。在此，我们报告参与该试验的小山羊的体液免疫和细胞介导免疫反应。接种疫苗和未接种疫苗的动物在受到刺激时均表现出γ干扰素刺激以及T细胞亚群的增殖。接种疫苗的山羊培养的外周血单核细胞中的CD4 +、CD25 +和γδ细胞在用抗原刺激时显示出明显更强的增殖反应。CD44 +细胞增加和CD62L +细胞减少表明疫苗接种降低了与感染相关的炎症反应。总体而言，与接种疫苗的山羊相比，感染山羊中观察到更强的抗体反应。采用两种独立的实验方法来鉴定接种疫苗和未接种疫苗的山羊抗体反应的差异。第一种方法涉及用感染山羊的混合血清筛选噬菌体表达文库，鉴定先前报道的抗原，包括MAP_1272c和MAP_1569。然而，在文库筛选中也鉴定出仅在接种疫苗的山羊中检测到的三种特异性抗原。使用斑点印迹分析的第二种方法在接种疫苗的山羊中鉴定出另外两种差异反应蛋白（MAP_4106和MAP_4141）。这些免疫学结果与先前获得的微生物学和病理学发现相结合，为接种疫苗的山羊副结核（Johne's）病控制提供了更完整的情况。
