Stokes A, Corteyn A H, Pullen L A, Doel T R, Meredith D M, Killington R A, Halliburton I W, Whittaker G R, Wheldon L A, Nicolson L
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
J Gen Virol. 1991 Apr;72 ( Pt 4):923-31. doi: 10.1099/0022-1317-72-4-923.
Hamsters were immunized with either an affinity-purified preparation of equid herpesvirus 1 (EHV-1) glycoprotein 13 (gp13) or synthetic peptides representing three sequences within the homologous glycoprotein of EHV-4, resulting in the production of anti-peptide (in the case of peptide-immunized animals) or antivirus antibodies. The sera from gp13-immunized hamsters contained antibodies which showed virus-neutralizing activity and complement-mediated antibody lysis of EHV-1-infected target cells. These hamsters were protected from EHV-1 challenge. The characteristics of a panel of anti-gp13 monoclonal antibodies (P28, P17, 14H7, 16E4 and 16H9) were assessed both in vivo and in vitro. 16E4 and P28 showed high levels of complement-mediated neutralization of virus, complement-mediated lysis of virus-infected target cells and passive protection of hamsters. Furthermore, epitope mapping studies demonstrated that this glycoprotein contains a neutralizing epitope recognized by EHV-1-immune horse serum. The data imply that gp13 has potential as a candidate antigen for a molecular vaccine.
用亲和纯化的马疱疹病毒1型（EHV-1）糖蛋白13（gp13）制剂或代表EHV-4同源糖蛋白内三个序列的合成肽对仓鼠进行免疫，从而产生抗肽抗体（在肽免疫动物的情况下）或抗病毒抗体。来自gp13免疫仓鼠的血清中含有具有病毒中和活性以及补体介导的对EHV-1感染靶细胞的抗体裂解作用的抗体。这些仓鼠受到了EHV-1攻击的保护。对一组抗gp13单克隆抗体（P28、P17、14H7、16E4和16H9）的特性进行了体内和体外评估。16E4和P28表现出高水平的补体介导的病毒中和作用、补体介导的对病毒感染靶细胞的裂解作用以及对仓鼠的被动保护作用。此外，表位作图研究表明，这种糖蛋白含有一个被EHV-1免疫马血清识别的中和表位。数据表明gp13有潜力作为分子疫苗的候选抗原。
