Guo P X, Goebel S, Davis S, Perkus M E, Languet B, Desmettre P, Allen G, Paoletti E
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
J Virol. 1989 Oct;63(10):4189-98. doi: 10.1128/JVI.63.10.4189-4198.1989.
The equine herpesvirus 1 (EHV-1) gene encoding glycoprotein 13 (gp13) was cloned into the hemagglutinin (HA) locus of vaccinia virus (Copenhagen strain). Expression of the gp13 gene was driven by the early/late vaccinia virus H6 promoter. Metabolically radiolabeled polypeptides of approximately 47 and 44 kilodaltons and 90 kilodaltons (glycosylated form) were precipitated with both polyclonal and gp13-specific monoclonal antibodies. Presentation of gp13 on the cytoplasmic membrane of cells infected with the recombinant gp13 vaccinia virus was demonstrated by immunofluorescence of unfixed cells. Inoculation of the recombinant gp13 vaccinia virus into guinea pigs induced neutralizing antibodies to both EHV-1 and vaccinia virus. Hamsters vaccinated with the recombinant gp13 vaccinia virus survived a lethal challenge with the hamster-adapted Kentucky strain of EHV-1. These results indicate that expression in vaccinia virus vectors of EHV-1 gp13, the glycoprotein homolog of herpes simplex virus gC-1 and gC-2, pseudorabies virus gIII, and the varicella-zoster virus gpV may provide useful vaccine candidates for equine herpesvirus infections.
将编码糖蛋白13（gp13）的马疱疹病毒1型（EHV-1）基因克隆到痘苗病毒（哥本哈根株）的血凝素（HA）基因座中。gp13基因的表达由早期/晚期痘苗病毒H6启动子驱动。用多克隆抗体和gp13特异性单克隆抗体沉淀出约47和44千道尔顿以及90千道尔顿（糖基化形式）的代谢放射性标记多肽。通过未固定细胞的免疫荧光证明了重组gp13痘苗病毒感染的细胞的细胞质膜上存在gp13。将重组gp13痘苗病毒接种到豚鼠体内可诱导产生针对EHV-1和痘苗病毒的中和抗体。用重组gp13痘苗病毒接种的仓鼠在接受适应仓鼠的EHV-1肯塔基株的致死性攻击后存活下来。这些结果表明，在痘苗病毒载体中表达EHV-1 gp13（单纯疱疹病毒gC-1和gC-2、伪狂犬病病毒gIII以及水痘-带状疱疹病毒gpV的糖蛋白同源物）可能为马疱疹病毒感染提供有用的候选疫苗。
