Afione S, Debeljuk L, Seilicovich A, Pisera D, Lasaga M, Díaz M C, Duvilanski B
Centro de Investigaciones en Reproducción, Facultad de Medicina Universidad de Buenos Aires, Argentina.
Peptides. 1990 Nov-Dec;11(6):1065-8. doi: 10.1016/0196-9781(90)90131-n.
In the present work we examined the effect of the neutralization of endogenous substance P by the administration of an anti-substance P serum (ASPS) on GABA concentration in the anterior pituitary in hyperprolactinemic conditions induced by 5-hydroxytryptophan or by grafting anterior pituitaries. ASPS reduced the increase in the anterior pituitary GABA concentration induced by hyperprolactinemia. In vitro experiments showed that substance P inhibited K(+)-evoked GABA efflux from hypothalamic fragments and decreased GABA concentration in the anterior pituitary but ASPS increased it. Our results demonstrate that substance P modifies hypothalamic GABA release and anterior pituitary GABA concentration and suggest that an interaction exists between substance P and GABA.
在本研究中，我们通过给予抗P物质血清（ASPS）来中和内源性P物质，研究其对由5-羟色氨酸或移植垂体前叶诱导的高催乳素血症状态下垂体前叶中γ-氨基丁酸（GABA）浓度的影响。ASPS降低了高催乳素血症诱导的垂体前叶GABA浓度的升高。体外实验表明，P物质抑制下丘脑碎片中钾离子诱发的GABA外流，并降低垂体前叶中的GABA浓度，但ASPS可使其升高。我们的结果表明，P物质可调节下丘脑GABA释放和垂体前叶GABA浓度，并提示P物质与GABA之间存在相互作用。
