Kim Ho-Bin, Park Min-Ju, Yang Hee-Chan, An Dong-Shan, Jin Hai-Zhu, Yang Deok-Chun
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, South Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, South Korea.
Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2529-2533. doi: 10.1099/ijs.0.64387-0.
A bacterial strain (designated KMY03T) that possesses beta-glucosidase activity was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. The bacterium was found to comprise Gram-negative, rod-shaped, motile cells with unipolar polytrichous flagella. On the basis of 16S rRNA gene sequence similarity, strain KMY03T was shown to belong to the family Burkholderiaceae of the Betaproteobacteria, being most closely related to Burkholderia caledonica LMG 19076T (97.8%), Burkholderia terricola LMG 20594T (97.5%), Burkholderia xenovorans LMG 21463T (97.4%) and Burkholderia phytofirmans LMG 22146T (97.3%). Chemotaxonomic data (major ubiquinone, Q-8; major fatty acids, C17:0 cyclo, C16:0, C19:0 cyclo omega8c and summed feature 2) supported the affiliation of the novel strain with the genus Burkholderia. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed the strain to be differentiated genotypically and phenotypically from Burkholderia species with validly published names. On the basis of these data, strain KMY03T represents a novel species of the genus Burkholderia, for which the name Burkholderia ginsengisoli sp. nov. is proposed. The type strain is KMY03T (=KCTC 12389T=NBRC 100965T).
从韩国人参田土壤中分离出一株具有β-葡萄糖苷酶活性的细菌菌株（命名为KMY03T），并对其进行了表征以确定其分类地位。该细菌为革兰氏阴性、杆状、具运动性的细胞，有单极多毛鞭毛。基于16S rRNA基因序列相似性，菌株KMY03T被证明属于β-变形菌纲的伯克霍尔德氏菌科，与喀里多尼亚伯克霍尔德氏菌LMG 19076T（97.8%）、土壤伯克霍尔德氏菌LMG 20594T（97.5%）、食 xenovorans伯克霍尔德氏菌LMG 21463T（97.4%）和植物伯克霍尔德氏菌LMG 22146T（97.3%）关系最为密切。化学分类数据（主要泛醌，Q-8；主要脂肪酸，C17:0环丙烷、C16:0、C19:0环丙烷ω8c和总和特征2）支持该新菌株与伯克霍尔德氏菌属的亲缘关系。DNA-DNA杂交以及生理生化测试结果使该菌株在基因型和表型上能够与已有效发表名称的伯克霍尔德氏菌属物种区分开来。基于这些数据，菌株KMY03T代表伯克霍尔德氏菌属的一个新物种，为此提出名为人参伯克霍尔德氏菌（Burkholderia ginsengisoli sp. nov.）。模式菌株为KMY03T（=KCTC 12389T=NBRC 100965T）。
