Sessitsch A, Coenye T, Sturz A V, Vandamme P, Barka E Ait, Salles J F, Van Elsas J D, Faure D, Reiter B, Glick B R, Wang-Pruski G, Nowak J
ARC Seibersdorf research GmbH, Department of Bioresources/Microbiology, A-2444 Seibersdorf, Austria.
Laboratory of Microbiology, Universiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1187-1192. doi: 10.1099/ijs.0.63149-0.
A Gram-negative, non-sporulating, rod-shaped, motile bacterium, with a single polar flagellum, designated strain PsJN(T), was isolated from surface-sterilized onion roots. This isolate proved to be a highly effective plant-beneficial bacterium, and was able to establish rhizosphere and endophytic populations associated with various plants. Seven related strains were recovered from Dutch soils. Based on 16S rRNA gene sequence data, strain PsJN(T) and the Dutch strains were identified as representing a member of the genus Burkholderia, as they were closely related to Burkholderia fungorum (98.7 %) and Burkholderia phenazinium (98.5 %). Analysis of whole-cell protein profiles and DNA-DNA hybridization experiments confirmed that all eight strains belonged to a single species. Strain PsJN(T) had a DNA G+C content of 61.0 mol%. Only low levels of DNA-DNA hybridization to closely related species were found. Qualitative and quantitative differences in fatty acid composition between strain PsJN(T) and closely related species were identified. The predominant fatty acids in strain PsJN(T) were 16 : 0, 18 : 1omega7c and summed feature 3 (comprising 16 : 1omega7c and/or iso-15 : 0 2-OH). Isolate PsJN(T) showed high 1-aminocyclopropane-1-carboxylate deaminase activity and is therefore able to lower the ethylene level in a developing or stressed plant. Production of the quorum-sensing signal compound 3-hydroxy-C8-homoserine lactone was detected. Based on the results of this polyphasic taxonomic study, strain PsJN(T) and the seven Dutch isolates are considered to represent a single, novel species, for which the name Burkholderia phytofirmans sp. nov. is proposed. The type strain is strain PsJN(T) (=LMG 22146(T) = CCUG 49060(T)).
从表面消毒的洋葱根部分离出一株革兰氏阴性、无芽孢、杆状、具运动性、有一根极生鞭毛的细菌，命名为菌株PsJN(T)。该分离株被证明是一种高效的植物有益细菌，能够在与多种植物相关的根际和内生菌群中定殖。从荷兰土壤中分离出7株相关菌株。基于16S rRNA基因序列数据，菌株PsJN(T)和荷兰菌株被鉴定为伯克霍尔德氏菌属的一个成员，因为它们与真菌伯克霍尔德氏菌（98.7%）和吩嗪伯克霍尔德氏菌（98.5%）密切相关。全细胞蛋白质谱分析和DNA-DNA杂交实验证实，所有8株菌株属于同一个种。菌株PsJN(T)的DNA G+C含量为61.0 mol%。仅发现与近缘种有低水平的DNA-DNA杂交。鉴定出菌株PsJN(T)与近缘种在脂肪酸组成上的定性和定量差异。菌株PsJN(T)中的主要脂肪酸为16 : 0、18 : 1ω7c和总和特征3（包含16 : 1ω7c和/或异-15 : 0 2-OH）。分离株PsJN(T)表现出高1-氨基环丙烷-1-羧酸脱氨酶活性，因此能够降低发育中的或受胁迫植物中的乙烯水平。检测到群体感应信号化合物3-羟基-C8-高丝氨酸内酯的产生。基于这一多相分类研究结果，菌株PsJN(T)和7株荷兰分离株被认为代表一个单一的新物种，为此提出新名称植物坚强伯克霍尔德氏菌（Burkholderia phytofirmans sp. nov.）。模式菌株为菌株PsJN(T)（=LMG 22146(T) = CCUG 49060(T)）。
