小鼠乳腺癌细胞中成纤维细胞生长因子（FGF）受体的表达性cDNA克隆：FGF反应性转化细胞中的一种变异形式

Expression cDNA cloning of fibroblast growth factor (FGF) receptor in mouse breast cancer cells: a variant form in FGF-responsive transformed cells.

作者信息

Kouhara H, Kasayama S, Saito H, Matsumoto K, Sato B

机构信息

Third Department of Internal Medicine, Osaka University Hospital, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Apr 15;176(1):31-7. doi: 10.1016/0006-291x(91)90885-b.

DOI:10.1016/0006-291x(91)90885-b

PMID:1708247

Abstract

Expression cDNA library of fibroblast growth factor (FGF)-responsive mouse breast cancer cells (SC-3) was prepared and screened using chick FGF receptor (FGF-R) cDNA as a probe. Two positive clones were isolated. Sequence analysis revealed that two clones have an identical full open reading frame. Compared with sequence data on mouse brain FGF-R, SC-3 cells were found to contain FGF-R with 12 amino acids insertion near N-terminal region. This insertion was mainly composed of hydrophobic amino acids. Additionally, the deletion of two amino acids in extracellular domain and the substitution of one amino acid in C-terminal region were identified in SC-3 cell FGF-R. Transfection of this clone into CHO cells resulted in a significant increase in basic FGF (bFGF) binding.

摘要

制备了成纤维细胞生长因子（FGF）反应性小鼠乳腺癌细胞（SC-3）的表达cDNA文库，并用鸡FGF受体（FGF-R）cDNA作为探针进行筛选。分离出两个阳性克隆。序列分析显示，两个克隆具有相同的完整开放阅读框。与小鼠脑FGF-R的序列数据相比，发现SC-3细胞在N端区域附近含有插入12个氨基酸的FGF-R。该插入主要由疏水氨基酸组成。此外，在SC-3细胞FGF-R中鉴定出细胞外结构域中两个氨基酸的缺失和C端区域中一个氨基酸的替换。将该克隆转染到CHO细胞中导致碱性FGF（bFGF）结合显著增加。