用电喷雾电离质谱法分析泪液葡萄糖浓度。
Analysis of tear glucose concentration with electrospray ionization mass spectrometry.
作者信息
Taormina Christopher R, Baca Justin T, Asher Sanford A, Grabowski Joseph J, Finegold David N
机构信息
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
出版信息
J Am Soc Mass Spectrom. 2007 Feb;18(2):332-6. doi: 10.1016/j.jasms.2006.10.002. Epub 2006 Nov 2.
Abstract
We have developed a mass spectrometry-based method that allows one to accurately determine the glucose concentration of tear fluid. We used a 1 microL micro-capillary to collect tear fluid from the tear meniscus with minimal irritation of the eye. We analyzed the 1 muL volume of collected tear fluid with liquid-chromatography electrospray ionization mass spectrometry with the use of D-glucose-6,6-d2 as an internal standard. Repeated measurements and a recovery experiment on pooled, onion-induced tears showed that the analysis of the glucose in tears was precise (4% relative standard deviation) and provided 100% recovery. We found the tear glucose concentration of one fasting nondiabetic subject to be 13 to 51 microM while the onion-induced tear glucose concentration of a different nondiabetic subject to be 211 to 256 microM.
摘要
我们开发了一种基于质谱的方法，可精确测定泪液中的葡萄糖浓度。我们使用1微升微量毛细管从泪液弯月面收集泪液，对眼睛的刺激最小。我们以D-葡萄糖-6,6-d2作为内标，采用液相色谱电喷雾电离质谱法分析1微升收集的泪液。对汇集的、洋葱诱导产生的泪液进行重复测量和回收率实验表明，泪液中葡萄糖的分析精确（相对标准偏差为4%），回收率为100%。我们发现一名空腹非糖尿病受试者的泪液葡萄糖浓度为13至51微摩尔，而另一名非糖尿病受试者洋葱诱导产生的泪液葡萄糖浓度为211至256微摩尔。
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