酶联免疫吸附测定（ELISA）与免疫测定法在检测人血清中针对西尼罗河病毒的IgG和IgM抗体并与病毒中和试验进行比较。
Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation.
作者信息
Niedrig M, Sonnenberg K, Steinhagen K, Paweska J T
机构信息
Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany.
出版信息
J Virol Methods. 2007 Jan;139(1):103-5. doi: 10.1016/j.jviromet.2006.09.009. Epub 2006 Nov 3.
Two commercial assays for the detection of IgG antibody to West Nile virus (WNV), an indirect enzyme-linked immunosorbent assay (I-ELISA) and indirect fluorescent antibody test (IFAT), were evaluated against the virus neutralisation test. Excellent agreement with the virus neutralisation was obtained with both tests, i.e., 99.5% by I-ELISA and 100% by IFAT. The well-known serological cross-reactivity within the family of the Flaviviridae was analysed using sera with known antibodies against dengue virus, tick borne encephalitis virus and yellow fever virus. IgM and/or IgG positive sera were examined for reactivity by WNV-ELISA and WNV-IFAT. While cross-reactivity between 0 and 18.2% was recorded with IgM positive sera, there was extensive cross-reactivity of 15.7-100% with IgG positive sera.
针对两种用于检测西尼罗河病毒（WNV）IgG抗体的商业检测方法，即间接酶联免疫吸附测定（I-ELISA）和间接荧光抗体试验（IFAT），与病毒中和试验进行了评估。两种检测方法与病毒中和试验的一致性都非常好，即I-ELISA为99.5%，IFAT为100%。使用针对登革病毒、蜱传脑炎病毒和黄热病病毒的已知抗体血清，分析了黄病毒科内众所周知的血清学交叉反应性。通过WNV-ELISA和WNV-IFAT检测IgM和/或IgG阳性血清的反应性。虽然IgM阳性血清的交叉反应率在0至18.2%之间，但IgG阳性血清的交叉反应率在15.7%至100%之间，范围广泛。
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