Sakamoto Tatsuhiko, Higaki Yasuki, Hara Megumi, Ichiba Masayoshi, Horita Mikako, Mizuta Toshihiko, Eguchi Yuichiro, Yasutake Tsutomu, Ozaki Iwata, Yamamoto Kyosuke, Onohara Shingo, Kawazoe Seiji, Shigematsu Hirohisa, Koizumi Shunzo, Tanaka Keitaro
Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga.
J Epidemiol. 2006 Nov;16(6):233-9. doi: 10.2188/jea.16.233.
The Ser326Cys polymorphism in human oxoguanine glycosylase 1 (hOGG1), which is involved in the repair of 8-hydroxy-2-deoxyguanine in oxidatively damaged DNA, has been associated with susceptibility to certain cancers, but has not been examined in causation of hepatocellular carcinoma (HCC).
We conducted a case-control study to investigate whether this polymorphism was related to HCC risk with any interaction with alcohol consumption and cigarette smoking. Genotyping was performed by a polymerase chain reaction with confronting two-pair primers among 209 newly diagnosed HCC cases, 275 hospital controls, and 381 patients with chronic liver disease (CLD) without HCC.
Overall, the hOGG1 genotype was not significantly associated with HCC; adjusted odds ratios (and 95% confidence intervals) for the Ser/Cys and Cys/Cys genotypes compared with the Ser/Ser genotype were 0.79 (0.35-1.79) and 0.48 (0.18-1.27) against hospital controls, and 1.51 (0.96-3.37) and 0.86 (0.50-1.47) against CLD patients. We could not detect any significant gene-alcohol interaction (p = 0.95 or 0.16) or gene-smoking interaction (p = 0.70 or 0.69).
These results suggest that the hOGG1 Ser326Cys polymorphism may not play a major role as an independent factor in hepatocarcinogenesis.
人类8-氧代鸟嘌呤糖苷酶1（hOGG1）中的Ser326Cys多态性参与氧化损伤DNA中8-羟基-2-脱氧鸟嘌呤的修复，与某些癌症的易感性有关，但尚未在肝细胞癌（HCC）的病因研究中进行过检测。
我们进行了一项病例对照研究，以调查这种多态性是否与HCC风险相关，以及是否与饮酒和吸烟存在任何相互作用。采用两对引物的聚合酶链反应对209例新诊断的HCC病例、275例医院对照和381例无HCC的慢性肝病（CLD）患者进行基因分型。
总体而言，hOGG1基因型与HCC无显著相关性；与Ser/Ser基因型相比，Ser/Cys和Cys/Cys基因型相对于医院对照的调整优势比（及95%置信区间）分别为0.79（0.35-1.79）和0.48（0.18-1.27），相对于CLD患者分别为1.51（0.96-3.37）和0.86（0.50-1.47）。我们未检测到任何显著的基因-酒精相互作用（p = 0.95或0.16）或基因-吸烟相互作用（p = 0.70或0.69）。
这些结果表明，hOGG1 Ser326Cys多态性可能不是肝癌发生中的一个独立主要因素。
