Micallef M, Hosokawa M, Shibata T, Nakane A, Yang Z B, Minagawa T, Kobayashi H
Laboratory of Pathology, Cancer Institute, Sapporo, Japan.
Cancer Immunol Immunother. 1991;33(1):33-8. doi: 10.1007/BF01742525.
We have studied the immunological effects that accompany a change in the chemical structure of a group of antineoplastic antibiotics by comparing the immunoregulatory cytokine release during mitogen-stimulated spleen cell culture after in vivo drug treatment. Whereas bleomycin and peplomycin increased cytokine levels in culture supernatants when compared with supernatants from untreated control rat spleen cell cultures, liblomycin generally reduced cytokine levels under the same culture conditions. We then compared these results with the antitumor effects of equivalent doses of the three drugs against a highly antigenic rat fibrosarcoma, KMT-17, both in vivo and in vitro. The results suggest that the immunoaugmenting effects of these antitumor antibiotics are essential for an optimal antitumor effect in vivo, and that these effects can be drastically altered by modification of the chemical structure of the drugs employed.
我们通过比较体内药物治疗后丝裂原刺激的脾细胞培养过程中免疫调节细胞因子的释放，研究了一组抗肿瘤抗生素化学结构变化所伴随的免疫效应。与未处理的对照大鼠脾细胞培养物的上清液相比，博来霉素和培洛霉素可提高培养上清液中的细胞因子水平，而在相同培养条件下，利布洛霉素通常会降低细胞因子水平。然后，我们将这些结果与等效剂量的这三种药物在体内和体外对高抗原性大鼠纤维肉瘤KMT-17的抗肿瘤作用进行了比较。结果表明，这些抗肿瘤抗生素的免疫增强作用对于体内最佳抗肿瘤效果至关重要，并且这些作用可因所用药物化学结构的改变而发生显著变化。
