Iñiguez C, De Juan J, al-Majdalawi A, Gayoso M J
Departamento de Histologia, Universidad de Alicante, Spain.
Brain Res Dev Brain Res. 1990 Dec 1;57(1):43-53. doi: 10.1016/0165-3806(90)90183-y.
This paper deals with the postnatal development of afferent and efferent connections of the rat striatum as revealed by the transport of horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP). Tracer was injected weekly from birth to the end of the first postnatal month in the head of the caudate nucleus. To control for transport from cortical areas contaminated by the micropipette, injections in newborn rats were made by either vertical or lateral penetrations. In addition some newborn and 14-day-old animals were injected only in the cortex. The results showed that at birth there was retrograde transport to the thalamus, substantia nigra and raphe nuclei. Labelling in the cortex was seen at birth but was probably due to cortical contamination. Transport from the striatum was clearly established on day 7, when a few labelled neurons were observed on both the ipsi and contralateral sides. These neurons increased in number and were distributed through layers III to VI by day 14. At this time labelled cell bodies were observed in the claustrum and lateral amygdaloid nucleus as well as in the globus pallidus and entopeduncular nucleus. On day 21 the contralateral labelling of the lateral amygdaloid nucleus was apparent. The anterograde transport from the striatum to globus pallidus, entopeduncular nucleus and substantia nigra was already visible at birth although its intensity increased during the first postnatal month.
本文研究了大鼠纹状体传入和传出连接的出生后发育情况，该研究通过与麦胚凝集素结合的辣根过氧化物酶（WGA-HRP）的运输来揭示。从出生到出生后第一个月末，每周将示踪剂注射到尾状核头部。为了控制来自微量移液器污染的皮质区域的运输，新生大鼠的注射通过垂直或横向穿刺进行。此外，一些新生和14日龄动物仅在皮质进行注射。结果表明，出生时存在向丘脑、黑质和中缝核的逆行运输。出生时在皮质可见标记，但可能是由于皮质污染。从纹状体的运输在第7天明确建立，此时在同侧和对侧均观察到一些标记神经元。到第14天，这些神经元数量增加并分布在III至VI层。此时，在屏状核、外侧杏仁核以及苍白球和内囊核中观察到标记的细胞体。在第21天，外侧杏仁核的对侧标记明显。从纹状体到苍白球、内囊核和黑质的顺行运输在出生时就已可见，尽管其强度在出生后第一个月有所增加。
