Yuan Y, Reddy R
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Biochim Biophys Acta. 1991 May 2;1089(1):33-9. doi: 10.1016/0167-4781(91)90081-v.
Human mitochondrial RNA processing (MRP) RNA is a 270 nucleotide-long small RNA found as ribonucleoprotein particles. In this study, we isolated four human genomic clones with homology to human MRP RNA. Two of these clones contained one copy each of the real gene coding for human MRP RNA; the other two clones represented a processed psuedogene. The Southern blot with the genomic DNA showed that the haploid human genome contains one copy of real gene and a few pseudogenes for MRP/7-2 RNA. The human MRP RNA is synthesized by RNA polymerase III and the 5' flanking sequences -84 to 1 of MRP RNA gene, containing TATA and PSE-like elements, are required and sufficient for transcription in vitro.
人线粒体RNA加工（MRP）RNA是一种长度为270个核苷酸的小RNA，以核糖核蛋白颗粒的形式存在。在本研究中，我们分离出四个与人MRP RNA具有同源性的人类基因组克隆。其中两个克隆各自包含一个编码人MRP RNA的真实基因拷贝；另外两个克隆代表一个加工过的假基因。用基因组DNA进行的Southern印迹显示，单倍体人类基因组包含一个真实基因拷贝以及一些MRP/7-2 RNA的假基因。人MRP RNA由RNA聚合酶III合成，MRP RNA基因5'侧翼序列-84至-1，包含TATA和类PSE元件，对于体外转录是必需且足够的。
