基于结核分枝杆菌苹果酸合酶和MPT51的血清学诊断检测作为快速诊断肺结核的辅助手段
Mycobacterium tuberculosis malate synthase- and MPT51-based serodiagnostic assay as an adjunct to rapid identification of pulmonary tuberculosis.
作者信息
Achkar Jacqueline M, Dong Yuxin, Holzman Robert S, Belisle John, Kourbeti Irene S, Sherpa Tsering, Condos Rany, Rom William N, Laal Suman
机构信息
New York University School of Medicine, New York, New York 10016, USA.
出版信息
Clin Vaccine Immunol. 2006 Nov;13(11):1291-3. doi: 10.1128/CVI.00158-06.
Abstract
The 81-kDa malate synthase (MS; Rv 1837c) and the 27-kDa MPT51 (Rv 3803c) of Mycobacterium tuberculosis are immunodominant antigens recognized by serum antibodies from approximately 80% of human immunodeficiency virus-negative smear-positive tuberculosis patients from India. We now provide evidence that the use of the MS/MPT51-based serodiagnostic assay can serve as an adjunct to sputum microscopy in the rapid diagnosis of pulmonary tuberculosis.
摘要
结核分枝杆菌的81 kDa苹果酸合酶（MS；Rv 1837c）和27 kDa的MPT51（Rv 3803c）是免疫显性抗原，约80%来自印度的人类免疫缺陷病毒阴性涂片阳性肺结核患者的血清抗体可识别这些抗原。我们现在提供证据表明，基于MS/MPT51的血清学诊断检测可作为痰涂片显微镜检查的辅助手段，用于快速诊断肺结核。
相似文献
1
Mycobacterium tuberculosis malate synthase- and MPT51-based serodiagnostic assay as an adjunct to rapid identification of pulmonary tuberculosis.基于结核分枝杆菌苹果酸合酶和MPT51的血清学诊断检测作为快速诊断肺结核的辅助手段
Clin Vaccine Immunol. 2006 Nov;13(11):1291-3. doi: 10.1128/CVI.00158-06.
2
Antibodies against immunodominant antigens of Mycobacterium tuberculosis in subjects with suspected tuberculosis in the United States compared by HIV status.按HIV感染状况比较美国疑似结核病患者体内抗结核分枝杆菌免疫显性抗原的抗体。
Clin Vaccine Immunol. 2010 Mar;17(3):384-92. doi: 10.1128/CVI.00503-09. Epub 2010 Jan 13.
3
Antibodies against Mycobacterial proteins as biomarkers for HIV-associated smear-negative tuberculosis.抗分枝杆菌蛋白抗体作为HIV相关涂片阴性结核病的生物标志物
Clin Vaccine Immunol. 2014 Jun;21(6):791-8. doi: 10.1128/CVI.00805-13. Epub 2014 Mar 26.
4
Biomarkers for clinical and incipient tuberculosis: performance in a TB-endemic country.用于临床和潜伏性结核病的生物标志物：在结核病流行国家的表现
PLoS One. 2008 Apr 30;3(4):e2071. doi: 10.1371/journal.pone.0002071.
5
Antibody response in pulmonary tuberculosis against recombinant 27kDa (MPT51, Rv3803c) protein of Mycobacterium tuberculosis.肺结核中针对结核分枝杆菌重组27kDa（MPT51，Rv3803c）蛋白的抗体反应。
Scand J Infect Dis. 2007;39(10):867-74. doi: 10.1080/00365540701402954.
6
Health care workers humoral immune response against GLcB, MPT51 and HSPX from Mycobacterium tuberculosis.结核分枝杆菌 GLcB、MPT51 和 HSPX 对卫生保健工作者体液免疫应答的影响
Braz J Infect Dis. 2009 Dec;13(6):417-21. doi: 10.1590/s1413-86702009000600006.
7
Antigens of Mycobacterium tuberculosis recognized by antibodies during incipient, subclinical tuberculosis.在初期亚临床结核病期间被抗体识别的结核分枝杆菌抗原。
Clin Diagn Lab Immunol. 2005 Feb;12(2):354-8. doi: 10.1128/CDLI.12.2.354-358.2005.
8
Comparative evaluation of profiles of antibodies to mycobacterial capsular polysaccharides in tuberculosis patients and controls stratified by HIV status.按HIV状态分层的结核病患者和对照中抗分枝杆菌荚膜多糖抗体谱的比较评估
Clin Vaccine Immunol. 2012 Feb;19(2):198-208. doi: 10.1128/CVI.05550-11. Epub 2011 Dec 14.
9
Humoral immune responses of tuberculosis patients in Brazil indicate recognition of Mycobacterium tuberculosis MPT-51 and GlcB.巴西结核病患者的体液免疫反应表明其对结核分枝杆菌MPT-51和GlcB具有识别能力。
Clin Vaccine Immunol. 2008 Mar;15(3):579-81. doi: 10.1128/CVI.00359-07. Epub 2008 Jan 16.
10
Humoral immune response in human pulmonary tuberculosis: antibodies against Mycobacterium tuberculosis polysaccharide, protein and glycolipid antigens.人类肺结核中的体液免疫反应：针对结核分枝杆菌多糖、蛋白质和糖脂抗原的抗体
Roum Arch Microbiol Immunol. 1993 Apr-Jun;52(2):109-19.
引用本文的文献
1
Accuracy of anti-mycobacterial protein 51 antibodies as a biomarker for latent TB infection in asymptomatic HIV positive individuals: a cross-sectional diagnostic study.抗分枝杆菌蛋白51抗体作为无症状HIV阳性个体潜伏性结核感染生物标志物的准确性：一项横断面诊断研究。
BMC Res Notes. 2025 Feb 17;18(1):73. doi: 10.1186/s13104-025-07134-1.
2
Editorial: Immuno-diagnosis of active tuberculosis; are we making progress?社论：活动性结核病的免疫诊断；我们有进展吗？
Front Immunol. 2023 Feb 24;14:1092651. doi: 10.3389/fimmu.2023.1092651. eCollection 2023.
3
Adhesion molecules facilitate host-pathogen interaction & mediate pathogenesis.黏附分子促进宿主-病原体相互作用并介导发病机制。
Indian J Med Res. 2019 Jul;150(1):23-32. doi: 10.4103/ijmr.IJMR_2055_16.
4
Design and Construction of a Eukaryotic Cloning Vector Encoding the mpt51 Gene of .编码[具体物种]mpt51基因的真核克隆载体的设计与构建 。（原文中“of.”后缺少具体物种信息）
Rep Biochem Mol Biol. 2019 Apr;8(1):32-35.
5
A Subgroup of Latently Mycobacterium tuberculosis Infected Individuals Is Characterized by Consistently Elevated IgA Responses to Several Mycobacterial Antigens.潜伏性结核分枝杆菌感染个体的一个亚组的特征是对几种分枝杆菌抗原的IgA反应持续升高。
Mediators Inflamm. 2015;2015:364758. doi: 10.1155/2015/364758. Epub 2015 Aug 10.
6
Identification of novel Mycobacterium tuberculosis CD4 T-cell antigens via high throughput proteome screening.通过高通量蛋白质组筛选鉴定新型结核分枝杆菌 CD4 T 细胞抗原。
Tuberculosis (Edinb). 2015 May;95(3):275-87. doi: 10.1016/j.tube.2015.03.001. Epub 2015 Mar 27.
7
Mycobacterium tuberculosis Secreted Proteins As Potential Biomarkers for the Diagnosis of Active Tuberculosis and Latent Tuberculosis Infection.结核分枝杆菌分泌蛋白作为活动性结核病和潜伏性结核感染诊断的潜在生物标志物
J Clin Lab Anal. 2015 Sep;29(5):375-82. doi: 10.1002/jcla.21782. Epub 2014 Aug 17.
8
Mycobacterium tuberculosis pili (MTP), a putative biomarker for a tuberculosis diagnostic test.结核分枝杆菌菌毛（MTP），一种潜在的结核病诊断测试生物标志物。
Tuberculosis (Edinb). 2014 May;94(3):338-45. doi: 10.1016/j.tube.2014.03.004. Epub 2014 Mar 20.
9
Antibodies against Mycobacterial proteins as biomarkers for HIV-associated smear-negative tuberculosis.抗分枝杆菌蛋白抗体作为HIV相关涂片阴性结核病的生物标志物
Clin Vaccine Immunol. 2014 Jun;21(6):791-8. doi: 10.1128/CVI.00805-13. Epub 2014 Mar 26.
10
Opportunities for improved serodiagnosis of human tuberculosis, bovine tuberculosis, and paratuberculosis.改进人类结核病、牛结核病和副结核病血清诊断的机会。
Vet Med Int. 2012;2012:674238. doi: 10.1155/2012/674238. Epub 2012 Jun 6.
本文引用的文献
1
Immunogenicity of the Mycobacterium tuberculosis PPE55 (Rv3347c) protein during incipient and clinical tuberculosis.结核分枝杆菌PPE55（Rv3347c）蛋白在初发和临床结核病期间的免疫原性。
Infect Immun. 2005 Aug;73(8):5004-14. doi: 10.1128/IAI.73.8.5004-5014.2005.
2
Expression and purification of recombinant 38-kDa and Mtb81 antigens of Mycobacterium tuberculosis for application in serodiagnosis.结核分枝杆菌重组38 kDa和Mtb81抗原的表达与纯化及其在血清学诊断中的应用
Protein Expr Purif. 2005 Mar;40(1):169-76. doi: 10.1016/j.pep.2004.10.016.
3
Antigens of Mycobacterium tuberculosis recognized by antibodies during incipient, subclinical tuberculosis.在初期亚临床结核病期间被抗体识别的结核分枝杆菌抗原。
Clin Diagn Lab Immunol. 2005 Feb;12(2):354-8. doi: 10.1128/CDLI.12.2.354-358.2005.
4
Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens.评估35种结核分枝杆菌蛋白的血清学诊断潜力并鉴定四种新型血清学抗原。
J Clin Microbiol. 2005 Jan;43(1):57-65. doi: 10.1128/JCM.43.1.57-65.2005.
5
Tuberculosis serodiagnosis in a predominantly HIV-infected population of hospitalized patients with cough, Botswana, 2002.2002年，在博茨瓦纳以咳嗽为主诉入院的主要感染艾滋病毒的人群中进行结核病血清学诊断。
Clin Infect Dis. 2004 Jul 1;39(1):e1-7. doi: 10.1086/421388. Epub 2004 Jun 14.
6
Cloning, expression, and purification of the 27 kDa (MPT51, Rv3803c) protein of Mycobacterium tuberculosis.结核分枝杆菌27 kDa（MPT51，Rv3803c）蛋白的克隆、表达及纯化
Protein Expr Purif. 2004 Jul;36(1):53-60. doi: 10.1016/j.pep.2004.01.016.
7
Potential serological use of a recombinant protein that is a replica of a Mycobacterium tuberculosis protein found in the urine of infected mice.一种重组蛋白在血清学方面的潜在用途，该重组蛋白是在受感染小鼠尿液中发现的结核分枝杆菌蛋白的复制品。
Clin Diagn Lab Immunol. 2004 Mar;11(2):280-6. doi: 10.1128/cdli.11.2.280-286.2004.
8
Combined use of serum and urinary antibody for diagnosis of tuberculosis.血清和尿液抗体联合用于结核病诊断
J Infect Dis. 2003 Aug 1;188(3):371-7. doi: 10.1086/376532. Epub 2003 Jul 15.
9
Isotype-specific anti-38 and 27 kDa (mpt 51) response in pulmonary tuberculosis with human immunodeficiency virus coinfection.人类免疫缺陷病毒合并感染的肺结核患者中同型特异性抗38 kDa和27 kDa（mpt 51）反应
Scand J Infect Dis. 2003;35(4):234-9. doi: 10.1080/00365540310000292.
10
The growing burden of tuberculosis: global trends and interactions with the HIV epidemic.结核病负担日益加重：全球趋势及与艾滋病流行的相互影响
Arch Intern Med. 2003 May 12;163(9):1009-21. doi: 10.1001/archinte.163.9.1009.
Zotero 插件