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FGFR-4，一种具有独特表达模式的新型酸性成纤维细胞生长因子受体。

FGFR-4, a novel acidic fibroblast growth factor receptor with a distinct expression pattern.

作者信息

Partanen J, Mäkelä T P, Eerola E, Korhonen J, Hirvonen H, Claesson-Welsh L, Alitalo K

机构信息

Department of Virology, University of Helsinki, Finland.

出版信息

EMBO J. 1991 Jun;10(6):1347-54. doi: 10.1002/j.1460-2075.1991.tb07654.x.

DOI:10.1002/j.1460-2075.1991.tb07654.x
PMID:1709094
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC452793/
Abstract

We have previously identified two novel members of the fibroblast growth factor receptor (FGFR) gene family expressed in K562 erythroleukemia cells. Here we report cDNA cloning and analysis of one of these genes, named FGFR-4. The deduced amino acid sequence of FGFR-4 is 55% identical with both previously characterized FGFRs, flg and bek, and has the structural characteristics of a FGFR family member including three immunoglobulin-like domains in its extracellular part. Antibodies raised against the carboxy terminus of FGFR-4 detected 95 and 110 kd glycoproteins with a protein backbone of 88 kd in COS cells transfected with a FGFR-4 cDNA expression vector. The FGFR-4 protein expressed in COS cells could also be affinity-labeled with radioiodinated acidic FGF. Furthermore, ligand binding experiments demonstrated that FGFR-4 binds acidic FGF with high affinity but does not bind basic FGF. FGFR-4 is expressed as a 3.0 kb mRNA in the adrenal, lung, kidney, liver, pancreas, intestine, striated muscle and spleen tissues of human fetuses. The expression pattern of FGFR-4 is distinct from that of flg and bek and the yet additional member of the same gene family, FGFR-3, which we have also cloned from the K562 leukemia cells. Our results suggest that FGFR-4 along with other fibroblast growth factor receptors performs cell lineage and tissue-specific functions.

摘要

我们之前在K562红白血病细胞中鉴定出成纤维细胞生长因子受体（FGFR）基因家族的两个新成员。在此我们报告其中一个名为FGFR - 4的基因的cDNA克隆及分析。FGFR - 4推导的氨基酸序列与之前已鉴定的FGFRs，即flg和bek，有55%的同源性，并且具有FGFR家族成员的结构特征，包括其胞外部分有三个免疫球蛋白样结构域。用针对FGFR - 4羧基末端产生的抗体，在转染了FGFR - 4 cDNA表达载体的COS细胞中检测到95 kd和110 kd的糖蛋白，其蛋白主链为88 kd。在COS细胞中表达的FGFR - 4蛋白也能用放射性碘化的酸性FGF进行亲和标记。此外，配体结合实验表明FGFR - 4与酸性FGF高亲和力结合，但不与碱性FGF结合。FGFR - 4在人类胎儿的肾上腺、肺、肾、肝、胰腺、肠、横纹肌和脾组织中作为3.0 kb的mRNA表达。FGFR - 4的表达模式与flg、bek以及我们同样从K562白血病细胞中克隆的同一基因家族的另一个成员FGFR - 3不同。我们的结果表明FGFR - 4与其他成纤维细胞生长因子受体一起发挥细胞谱系和组织特异性功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/edb22a26a17e/emboj00104-0056-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/57eafd8d2042/emboj00104-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/90a95fbcf094/emboj00104-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/edb22a26a17e/emboj00104-0056-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/57eafd8d2042/emboj00104-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/90a95fbcf094/emboj00104-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2afe/452793/edb22a26a17e/emboj00104-0056-b.jpg

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1
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.真核生物mRNA翻译起始位点上游序列的汇编与分析。
Nucleic Acids Res. 1984 Jan 25;12(2):857-72. doi: 10.1093/nar/12.2.857.
3
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
6-氨基-2,4,5-三甲基-3-吡啶醇和 2-氨基-4,6-二甲基-5-嘧啶醇衍生物作为选择性成纤维细胞生长因子受体 4 抑制剂的设计、合成、分子对接和抗肝癌疗效评价。
J Enzyme Inhib Med Chem. 2022 Dec;37(1):844-856. doi: 10.1080/14756366.2022.2048378.
4
Fibroblast growth factor receptor signalling dysregulation and targeting in breast cancer.成纤维细胞生长因子受体信号失调与乳腺癌靶向治疗。
Open Biol. 2022 Feb;12(2):210373. doi: 10.1098/rsob.210373. Epub 2022 Feb 23.
5
Functional Roles of FGF Signaling in Early Development of Vertebrate Embryos.FGF 信号在脊椎动物胚胎早期发育中的功能作用。
Cells. 2021 Aug 20;10(8):2148. doi: 10.3390/cells10082148.
6
Oncogenic FGFR Fusions Produce Centrosome and Cilia Defects by Ectopic Signaling.致癌性 FGFR 融合通过异位信号产生中心体和纤毛缺陷。
Cells. 2021 Jun 9;10(6):1445. doi: 10.3390/cells10061445.
7
Fibroblast Growth Factor Receptors (FGFRs) and Noncanonical Partners in Cancer Signaling.成纤维细胞生长因子受体（FGFRs）与癌症信号转导中的非经典伙伴。
Cells. 2021 May 14;10(5):1201. doi: 10.3390/cells10051201.
8
FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer.FGFR4抑制剂BLU9931可减弱胰腺癌细胞的增殖和侵袭，同时诱导衰老：胰腺癌中衰老溶解疗法潜力的证据。
Cancers (Basel). 2020 Oct 14;12(10):2976. doi: 10.3390/cancers12102976.
9
Fibroblast growth factor signalling in osteoarthritis and cartilage repair.成纤维细胞生长因子信号在骨关节炎和软骨修复中的作用。
Nat Rev Rheumatol. 2020 Oct;16(10):547-564. doi: 10.1038/s41584-020-0469-2. Epub 2020 Aug 17.
10
FGFR4: A promising therapeutic target for breast cancer and other solid tumors.成纤维细胞生长因子受体 4：乳腺癌和其他实体瘤有前途的治疗靶点。
Pharmacol Ther. 2020 Oct;214:107590. doi: 10.1016/j.pharmthera.2020.107590. Epub 2020 May 31.
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
4
Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.新型高效细菌表达载体家族的构建：编码人肝蛋白的cDNA克隆的鉴定
EMBO J. 1984 Jun;3(6):1429-34. doi: 10.1002/j.1460-2075.1984.tb01988.x.
5
Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.用二乙氨基乙基葡聚糖增强猿猴病毒40脱氧核糖核酸的感染性。
J Natl Cancer Inst. 1968 Aug;41(2):351-7.
6
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.肝素结合生长因子诱导非洲爪蟾早期胚胎的中胚层形成
Nature. 1987;326(6109):197-200. doi: 10.1038/326197a0.
7
Human basic fibroblast growth factor: nucleotide sequence and genomic organization.人碱性成纤维细胞生长因子：核苷酸序列与基因组结构
EMBO J. 1986 Oct;5(10):2523-8. doi: 10.1002/j.1460-2075.1986.tb04530.x.
8
Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization.人内皮细胞生长因子：克隆、核苷酸序列及染色体定位。
Science. 1986 Aug 1;233(4763):541-5. doi: 10.1126/science.3523756.
9
The immunoglobulin superfamily--domains for cell surface recognition.免疫球蛋白超家族——细胞表面识别结构域
Annu Rev Immunol. 1988;6:381-405. doi: 10.1146/annurev.iy.06.040188.002121.
10
Acidic fibroblast growth factor stimulates adrenal chromaffin cells to proliferate and to extend neurites, but is not a long-term survival factor.
Neuron. 1988 Nov;1(9):783-90. doi: 10.1016/0896-6273(88)90126-2.