Chaki Takamitsu, Kakimi Hideto, Shibata Akihiro, Baba Takashi
Central Research Institute, Maruha Group Inc., Tsukuba, Ibaraki, Japan.
Biosci Biotechnol Biochem. 2006 Nov;70(11):2793-6. doi: 10.1271/bbb.60313. Epub 2006 Nov 7.
We attempted in this study to detect alginate oligosaccharides (AO) from mollusks. The samples used were digestive organs taken from turban shells and abalones which commonly ate brown algae. High-performance liquid chromatography (HPLC) and negative-ion electrospray ionization (ESI) quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) analyses were used to confirm the presence of AO. Samples spiked with AO resulted in observable peaks where the HPLC area was increased. The highest content was estimated to be 401.8 mg/100 g of digestive organ. The product-ion data derived from AO molecular weight were detected at a constant interval by Q-TOF MS/MS analysis. These findings indicate that AO was present in the digestive organs of mollusks.
在本研究中，我们尝试从软体动物中检测海藻酸盐寡糖（AO）。所用样本是取自通常以褐藻为食的蝾螺和鲍鱼的消化器官。采用高效液相色谱（HPLC）和负离子电喷雾电离（ESI）四极杆飞行时间（Q-TOF）质谱（MS）分析来确认AO的存在。添加了AO的样本出现了可观察到的峰，HPLC面积增加。估计最高含量为401.8毫克/100克消化器官。通过Q-TOF MS/MS分析以恒定间隔检测到源自AO分子量的产物离子数据。这些发现表明AO存在于软体动物的消化器官中。
