Martinez-Garcia F, Olucha F E, Teruel V, Lorente M J, Schwerdtfeger W K
Universitat de Valéncia, Facultat de Ciéncies Biológiques, Departament de Biologia Animal, Unitat de Morfologia Microscópica, Spain.
J Comp Neurol. 1991 Mar 8;305(2):337-47. doi: 10.1002/cne.903050214.
The connections of the olfactory bulbs of Podarcis hispanica were studied by tract-tracing of injected horseradish peroxidase. Restricted injections into the main olfactory bulb (MOB) resulted in bilateral terminallike labeling in the medial part of the anterior olfactory nucleus (AON) and in the rostral septum, lateral cortex, nucleus of the lateral olfactory tract, and ventrolateral amygdaloid nucleus. Bilateral retrograde labeling was found in the rostral lateral cortex and in the medial and dorsolateral AON. Ipsilaterally the dorsal cortex, nucleus of the diagonal band, lateral preoptic area, and dorsolateral amygdala showed labeled cell bodies. Retrogradely labeled cells were also found in the midbrain raphe nucleus. Results from injections into the rostral lateral cortex and lateral olfactory tract indicate that the mitral cells are the origin of the centripetal projections of the MOB. Injections in the accessory olfactory bulb (AOB) produced ipsilateral terminallike labeling of the ventral AON, bed nucleus of the accessory olfactory tract, central and ventromedial amygdaloid nuclei, medial part of the bed nucleus of the stria terminalis, and nucleus sphericus. Retrograde labeling of neurons was observed ipsilaterally in the bed nucleus of the accessory olfactory tract and stria terminalis, in the central amygdaloid nucleus, dorsal cortex, and nucleus of the diagonal band. Bilateral labeling of somata was found in the ventral AON, the nucleus sphericus (hilus), and in the mesencephalic raphe nucleus and locus coeruleus. Injections into the dorsal amygdala showed that the mitral neurons are the cells of origin of the AOB centripetal projections. Reciprocal connections are present between AOB and MOB. To our knowledge, this is the first study to address the afferent connections of the olfactory bulbs in a reptile. On the basis of the available data, a discussion is provided of the similarities and differences between the reptilian and mammalian olfactory systems, as well as of the possible functional role of the main olfactory connections in reptiles.
通过注射辣根过氧化物酶进行束路追踪，研究了西班牙壁蜥嗅球的连接情况。向主嗅球（MOB）进行局限性注射，导致在前嗅核（AON）内侧部分、 Rostral隔、外侧皮质、外侧嗅束核和腹外侧杏仁核出现双侧终末样标记。在 Rostral外侧皮质以及AON内侧和背外侧发现双侧逆行标记。同侧的背侧皮质、斜角带核、外侧视前区和背外侧杏仁核显示有标记的细胞体。在中脑缝核中也发现了逆行标记的细胞。向 Rostral外侧皮质和外侧嗅束注射的结果表明，二尖瓣细胞是MOB向心投射的起源。向副嗅球（AOB）注射产生同侧腹侧AON、副嗅束床核、中央和腹内侧杏仁核、终纹床核内侧部分以及球状核的终末样标记。在同侧的副嗅束和终纹床核、中央杏仁核、背侧皮质和斜角带核中观察到神经元的逆行标记。在腹侧AON、球状核（hilus）以及中脑缝核和蓝斑中发现了双侧躯体标记。向背侧杏仁核注射表明，二尖瓣神经元是AOB向心投射的起源细胞。AOB和MOB之间存在相互连接。据我们所知，这是第一项研究爬行动物嗅球传入连接的研究。根据现有数据，讨论了爬行动物和哺乳动物嗅觉系统之间的异同，以及爬行动物主要嗅觉连接的可能功能作用。
